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A kind of preparation method of non-denatured jellyfish collagen

A collagen, non-denatured technology, applied in the field of preparation of non-denatured collagen, can solve the problems of high cost, unfavorable industrial promotion, low collagen yield, etc., and achieve the effect of complete structure, high purity and high extraction rate

Active Publication Date: 2019-04-02
原海生物(大连)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Among the existing collagen preparation techniques, acid extraction and enzymatic methods are the most widely used, but there are still two problems in these techniques: on the one hand, the yield of collagen is not high, and the acid extraction The collagen yield generally does not exceed 5%, some raw materials do not even contain acid-soluble collagen, and the collagen yield of the enzymatic method is also below 80%. On the other hand, the reaction temperature used in the enzymatic method is all below 10°C, which is expensive The extraction time is also more than 1 day, which is not conducive to industrial promotion

Method used

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  • A kind of preparation method of non-denatured jellyfish collagen
  • A kind of preparation method of non-denatured jellyfish collagen
  • A kind of preparation method of non-denatured jellyfish collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The pretreatment of pickled jellyfish processing leftovers

[0032] (1) Desalination: Rinse the marinated jellyfish processing scraps with clean water several times, then soak them in distilled water, and replace the distilled water every 2 hours for 1-2 days;

[0033] (2) Homogenization: Keep a certain proportion of water, grind it with a mixer until there are no obvious large particles, and then filter and drain with four layers of gauze;

[0034] (3) Degreasing: add acetone to a ratio of material to liquid of 1:5~1:20, let stand overnight, wash with a large amount of distilled water until there is no peculiar smell, then filter and drain with four layers of gauze;

[0035] (4) Impurity protein removal: Soak in 5-20 times the volume of 0.01-0.1 mol / L disodium hydrogen phosphate solution for 2-3 days, replace the solution every 6 hours, wash with a large amount of distilled water until neutral, and then use Filter and drain with four layers of gauze, stor...

Embodiment 2

[0036] Example 2 Optimization of jellyfish collagen extraction process

[0037] (1) Determination of collagen extraction rate: adopt the determination method of hydroxyproline in the national standard GB / T 9695.23-2008, indirectly quantify collagen through hydroxyproline content, and convert collagen to hydroxyproline conversion coefficient for 11.1. With the concentration of the standard L-hydroxyproline (μg / mL) as the abscissa and the absorbance A560 as the ordinate, draw a standard curve to obtain a linear regression equation A560 = 0.2193C+ 0.011, R = 0.9993;

[0038] (2) Determination of the integrity of the collagen triple helix structure: SDS-PAGE was used to analyze whether the collagen triple helix structure was destroyed. The separating gel concentration is 7.5%, and the stacking gel concentration is 3.5%;

[0039] (3) Determination of the best acid type: different extractants (glacial acetic acid, hydrochloric acid , citric acid and lactic acid) on collagen extra...

Embodiment 3

[0048] Example 3 Separation and purification of jellyfish collagen

[0049] (1) Slowly add high-concentration NaCl solution to the crude jellyfish collagen extract to a final concentration of 1.0 mol / L, and stir overnight;

[0050] (2) The crude extract was centrifuged at 20,000 g for 15 min at 4°C, and the precipitate was redissolved in 1 mmol / L citric acid, transferred to a 40 kDa dialysis bag, and dialyzed in distilled water for 3 days. Replace with distilled water once every 6 h;

[0051] (3) The dialysate was centrifuged at 20,000 g for 15 min at 4°C, and the precipitate was freeze-dried to obtain dry collagen cotton.

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Abstract

The invention provides a non-degenerated jellyfish collagen preparation method and belongs to the field of biotechnology. According to the method, processing leftovers of marinated jellyfish serve as raw materials, a non-degenerated jellyfish collagen extraction process under the medium-low temperature condition and with citric acid as extracting agents is established, a collagen product with high purity is obtained, the additional value of jellyfish products is effectively improved, high-valued utilization of low-value jellyfish is achieved, and a new source is provided for preparation of collagen.

Description

technical field [0001] The invention discloses a method for preparing non-denatured collagen by using leftovers of pickled jellyfish processing as raw materials, and relates to the field of food biotechnology. The invention has simple process and low cost, and the prepared collagen protein has high extraction rate and complete structure, is suitable for industrial production, and can be used in the fields of medicine, biological materials, beauty treatment and the like. technical background [0002] Collagen is the most abundant structural protein in organisms. It is widely distributed in connective tissues such as mammalian skin, bone, blood vessels and tendons, and plays the role of support, repair and protection. Collagen contains a unique triple helical structure composed of three α-peptide chains, generally with relatively large molecular weight, insoluble in water and dilute alkali, and partially soluble in dilute acid or neutral salt to some extent. The helical struc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/34
Inventor 林娟郑明星黄新华朱凡叶秀云
Owner 原海生物(大连)有限公司
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