Detection kit and detection method

Abstract

A detection kit applied for detecting a body fluid sample of an organism includes a sampling device and a detecting device. The sampling device includes a sampling unit and a filtering unit detachably covering the sampling unit. The sampling unit collects the body fluid sample that is filtered by the filtering unit. The detecting device includes at least one reaction zone including a fiber substrate and a detecting reagent immobilized to the fiber substrate. The reaction zone is disposed as the fiber substrate contacts the body fluid sample collected by the sampling unit. The fiber substrate absorbs the body fluid sample collected by the sampling unit, so that the body fluid sample reacts with the detecting reagent for the detection. A detection method for detecting a body fluid sample is also disclosed. This invention is advantageous for simplified pretreatment, less amount of the reagent and rapid analysis.

Description

Technical field [0001] The invention relates to a detection kit and a detection method, in particular to a detection kit and a detection method for biomedical detection. Background technique [0002] In clinical practice, specimen sampling instruments used for biological bodies generally include brush head type, film type, curette type, etc. Taking the detection of endometrial cancer specimens as an example, the method of use is to place the specimen sampling instrument on the specimen to be sampled, and use suction or scraping of the endometrial surface to obtain endometrial cells and their secretions. Cells and their secretions are processed and tested; there are many clinical applications that use sample sampling devices to take, for example, body fluids from the affected part of the organism to determine the disease process or treatment effect. However, the samples sampled by the above-mentioned traditional sampling devices often contain many unnecessary non-detectable subst...

AI Technical Summary

Problems solved by technology

However, the traditional ELISA detection using a 96-well plate requires about 50-100 microliters of analytical samples and reagents each time; in addition, the reagents and samples need to be reacted at a specific temperature (such as 37°C), and repeated Perform the reaction and washing steps, each step takes about tens of minutes to one hour, and finally use the spectrometer to measure the absorbance value to obtain the analysis results, the operation steps are complicated and time-consuming

Although the use of Dot-ELISA (also known as Dot-Blot) detection method can reduce the amount of analysis samples and reagents required, it still requires time-consuming reaction and washing steps

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