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Application of deoxyschizandrin to preparation of medicine for treating diabetes

The technology of Schisandrin A and a diabetes drug is applied in the field of medicine, and can solve the problems of lowering blood sugar and anti-diabetic effects without any reports, and achieves the effects of lowering blood sugar level and high sensitivity of action.

Inactive Publication Date: 2016-01-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Schisandra chinensis is contained in many traditional Chinese medicines for treating diabetes. Some studies have shown that the extract of schisandra chinensis has the effect of lowering blood sugar, but there is no report on the anti-diabetic effect of the monomer components in schisandra chinensis

Method used

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  • Application of deoxyschizandrin to preparation of medicine for treating diabetes
  • Application of deoxyschizandrin to preparation of medicine for treating diabetes
  • Application of deoxyschizandrin to preparation of medicine for treating diabetes

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Study on the Hypoglycemic Activity of 5μM Schizandrin A Liquid on Insulin-resistant Hepatocytes

[0023] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture medi...

Embodiment 2

[0028] Study on the Hypoglycemic Activity of 10μM Schizandrin A Liquid on Insulin-resistant Hepatocytes

[0029] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture med...

Embodiment 3

[0034] Study on the Hypoglycemic Activity of 20μM Schizandrin A Liquid on Insulin-resistant Hepatocytes

[0035] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture med...

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Abstract

The invention discloses application of deoxyschizandrin to preparation of a medicine for treating diabetes and provides a pharmaceutical composition taking the deoxyschizandrin as an active ingredient. The pharmaceutical composition is capable of decreasing blood sugar remarkably and is far higher than first-line medicine metformin in the market in acting sensitivity.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a medicine for preventing and treating diabetes, in particular to the application of schisandrin A in the preparation of medicine for preventing and treating diabetes. Background technique [0002] According to the statistics of the International Diabetes Federation (International Diabetes Federation, IDF), there were 387 million diabetics in the world in 2014, and the global incidence rate was 8.3%. It is estimated that by 2035, the number of diabetics in the world will reach 592 million. The Diabetes Branch of the Chinese Medical Association conducted a diabetes epidemiological survey in some areas of my country from 2007 to 2008, showing that the prevalence of diabetes among people over 20 years old in my country was 9.7%, and the proportion of prediabetes was 15.5%. Diabetes is a chronic disease. With the prolongation of the disease course, various metabolic disorders can lead to ...

Claims

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Application Information

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IPC IPC(8): A61K31/09A61P3/10
Inventor 程翼宇王毅范骁辉
Owner ZHEJIANG UNIV
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