Microarray hybridization elution instrument

Abstract

A biochip microarray hybridization elution instrument is composed of a hybridization instrument, an elution instrument, accessories hybridization boxes, washing liquid bottles and a waste liquid bottle and is used for completion of microarray hybridization elution. The hybridization instrument can be used for setting the temperature and the time, six hybridization boxes are placed at the same time, and left and right oscillation can be performed after a power supply is switched on; the elution instrument adopts pipeline heating and can automatically set the elution temperature, the eluant amount, the number of elution times and the elution time. Needles are arranged on a needle seat directly facing to the upper side of an object stage, a group is composed of two needles, the needles totally comprise 20 groups, each 10 groups form a unit, and the units can be independently controlled. The two needles are both hollow, one is used for adding an eluant in the washing liquid bottles, and the other one is used for taking out waste liquid in the waste liquid bottle. The upper side of each reaction chamber of an upper cover of each hybridization box is provided with a sample adding hole in accordance with the position of the needle on the elution instrument. The full set of device is easy to use, after hybridization, elution is completed under the condition without disassembly of the hybridization boxes and chips, cross contamination is not generated, and the device is a good microarray hybridization elution device.

Description

technical field [0001] The invention relates to a microarray hybridization and cleaning device, which is used for the hybridization and post-hybridization elution of the biochip microarray. Background technique [0002] For biochip microarrays, the chip is usually placed statically under the condition of constant temperature and humidity for hybridization reaction. This method is not conducive to the flow of hybridization solution, which will lead to uneven hybridization of the entire chip, making the error of repeated determination between chips larger. Large, not conducive to repeated experiments and quantitative analysis. Later, a hybridization instrument that can move back and forth with a certain amplitude appeared. The carrying platform of this kind of equipment can perform specific regular movements, which can effectively promote the flow of the hybridization solution in the hybridization box. However, there are still some deficiencies, such as: the equipment itself ...

AI Technical Summary

Problems solved by technology

Since the hybridization and elution equipment are all transported by pipelines, the two-step integrated equipment is likely to cause liquid residues that cannot be cleaned in the pipelines, resulting in cross-contamination

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