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A kind of artificial breeding method of cotton aphid parasitoid - Aphidia gossypii parasitoid

A kind of Aphids gossypii, artificial technology, applied in the field of artificial breeding of cotton aphid parasitoids, can solve the problems of cotton aphids not being collected, aphids running and crawling, loss of Aphids gossypii, shorten the development cycle, parasitize The effect of high rate and control of bee-out time

Inactive Publication Date: 2017-10-31
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the artificial breeding method of Aphids gossypii. The closest method is the breeding method of Aphids aphids. The method used is to directly graft adult aphids on the seedlings, and after a few days, the adult aphids will breed small aphids. Re-introduction of parasitoids, because parasitoids have a certain selectivity to the age of the host, resulting in a low parasitism rate
Due to its small size, its host aphids can secrete honeydew. Putting Aphids gossypii in beekeeping cages for parasitism, the parasitism rate is very low, and part of Aphis gossypii is secreted by aphids at the bottom of beekeeping cages The honeydew sticks to death, causing a serious loss of Aphids gossypii, and the existing large-area breeding method is not suitable for indoor small-area artificial breeding
Moreover, after the cotton aphid is parasitized, it will send out an alarm pheromone, causing the aphid to run around and crawl around, and the parasitized cotton aphid cannot be collected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Artificial rearing method of cotton aphid cocoon

[0023] The method includes the following steps:

[0024] 1. Take the dead aphid from the field and put it in a beekeeping cage with flowering zucchini for the cotton aphid to feed nectar. At the same time, 25% honey-moistened absorbent cotton is placed in the beekeeping cage for the upper part of the beekeeping cage. Seal with filter paper and put a layer of filter paper at the bottom. One day after the dead aphids emerge from the bees, the female and male bees mate naturally, and the adult bees after mating are put into the fresh-keeping box with aphids the next day. Moisten the bottom of the fresh-keeping box with water, spread cotton leaves in the fresh-keeping box, connect with 2 to 3 instar aphids, and access the cotton aphid cocoon at a ratio of 1:50 (ie, cotton aphid cocoon wasp: cotton aphid = 1:50). Put 25% honey-moistened absorbent cotton in the fresh-keeping box, seal the top of the fresh-keeping box wi...

Embodiment 2

[0032] Example 2 Constant-temperature feeding test of Aphis gossypii

[0033] 1. Take the dead aphid from the field and put it into the beekeeping cage with flowering zucchini for the cotton aphid to feed the nectar. At the same time, 25% honey-moist absorbent cotton is placed. The top of the beekeeping cage is sealed with filter paper, and the bottom is Put a layer of filter paper, one day after the dead aphids emerge from the bees, the female bees and the male bees mate naturally, and the adult bees after mating are put into the fresh-keeping box with aphids the next day.

[0034] 2. After 12 hours of parasitism, the parasitic wasps can be removed, and the parasitic wasps can be re-inserted in a new storage box; the double-layer cotton leaf method is used to raise the parasitic aphids, and the breeding conditions are 14 hours of light / darkness 10h, temperature 26℃, humidity 80%.

[0035] 3. After the parasitic aphids are reared at 26℃ for 4-5 days, that is, when most of the aphids...

Embodiment 3

[0042] Example 3 Experiments on Breeding Cotton Aphids without Double Cotton Leaf Method

[0043] 1. Take the dead aphid from the field and put it into the beekeeping cage with flowering zucchini for the cotton aphid to feed the nectar. At the same time, 25% honey-moist absorbent cotton is placed. The top of the beekeeping cage is sealed with filter paper, and the bottom is Put a layer of filter paper, one day after the dead aphids emerge from the bees, the female bees and the male bees mate naturally, and the adult bees after mating are put into the fresh-keeping box with aphids the next day.

[0044] 2. After 12 hours of parasitism, the parasitic wasps can be removed, and the parasitic wasps can be re-inserted in a new fresh-keeping box; the parasitized cotton aphids are kept in the fresh-keeping box, and the fresh-keeping box contains 18% The agar medium was covered with a layer of cotton leaves, and the fresh-keeping box was sealed with plastic wrap and pierced with dense small...

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PUM

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Abstract

The invention provides an artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica. According to the method, on the basis of biological characteristics of the Lysiphlebia japonica and cotton aphids, the Lysiphlebia japonica is inoculated with aphids at the preference age of the Lysiphlebia japonica, the Lysiphlebia japonica is fed through stepped temperature change, the parasitized aphids are fed with a double-layer cotton leaf method, the phenomenon that the parasitized aphids run and climb about due to the fact that the aphids give out alarm pheromones is avoided, the emergence time of the Lysiphlebia japonica can be effectively controlled, the growth period of the Lysiphlebia japonica can be shortened, and the lives of adult Lysiphlebia japonica can be prolonged. When the Lysiphlebia japonica is fed with the method, the parasitic rate of the Lysiphlebia japonica is high, the loss is low, the Lysiphlebia japonica is fed step by step, and feeding conditions can be controlled step by step. The method is convenient to operate and low in cost, indoor continuous feeding of the Lysiphlebia japonica can be realized, and a good foundation is laid for large-scale propagation and feeding of the Lysiphlebia japonica.

Description

Technical field [0001] The invention relates to a method for artificial breeding of cotton aphid parasitic wasps, in particular to a method for artificial breeding of cotton aphid parasitic wasps. Background technique [0002] The cotton aphid (Aphis gossypii Glover) is a common pest widely distributed in various countries in the world. According to records, there are 76 families of 600 species of host plants of cotton aphid in the world, among which cotton and cucurbit melons are the main expatriate hosts (second host, summer host) of cotton aphid (Zhang Guangxue and Zhong Tiesen, 1983; Blackman, 2000) ). Because the cotton aphid has a short reproductive cycle and large reproduction volume, once the conditions are suitable, it can break out in a short period of time. In addition, cotton aphids cause harm through migration, spreading and feeding, and at the same time spread viral diseases of various vegetable crops such as melons. Especially in recent years, with the widesprea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 高雪珂崔金杰张帅吕丽敏王春义雒珺瑜李春花
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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