Comprehensive identification and evaluation method for bitter gourd cold resistance

Abstract

The invention discloses a comprehensive identification and evaluation method for bitter gourd cold resistance. The method includes the steps that germination energy and the activity index of seeds, the seedling cold damage index and the pollen germination rate under the low-temperature condition are measured, and bitter gourd cold resistance is comprehensively identified and evaluated by means of principal component analysis, subordinative function value analysis and cluster analysis. Several fuzzy evaluation methods are comprehensively used, a fuzzy evaluation object is processed through accurate digital measures, and materials containing information presenting fuzziness can be quantitatively evaluated scientifically and reasonably in the mode of being close to reality. As the indexes related to a bud stage, a seedling stage and a fruit-bearing pollination stage are adopted at the same time, when the comprehensive identification and evaluation method is used for carrying out tolerance identification on a great number of materials, the tolerance essence of bitter gourd crops can be really reflected, accuracy and reliability of identification are improved, and the comprehensive identification and evaluation method is suitable for comprehensively identifying and evaluating cold resistance of different bitter gourd resources, which is beneficial for accelerating bitter gourd cold resisting seed breeding.

Description

technical field [0001] The invention belongs to the technical field of identification and evaluation of germplasm resources of bitter gourd, in particular to a method for comprehensive identification and evaluation of cold tolerance of bitter gourd. Background technique [0002] Bitter gourd (Momordicacharantia L.) originated in the tropical region of Southeast Asia and is widely distributed in tropical, subtropical and temperate regions. Pollen germination was inhibited at 15℃. In early spring cultivation, low temperature accompanies each growth stage of bitter gourd. To essentially solve the problem of chilling damage in production, it is still necessary to carry out cold-resistant breeding of bitter gourd. [0003] At present, the identification and evaluation of cold tolerance of bitter gourd are mostly focused on the seedling and bud stages, and the pollination and fruiting stages are less involved. The direct identification method is mostly used, and the indirect meth...

AI Technical Summary

Problems solved by technology

The direct identification method is limited by natural conditions and the identification cost is high; the indirect identification index is single, and it is difficult to truly reflect the essence of plant tolerance. accuracy, reliability

The stress tolerance of plants is a quantitative trait controlled by multiple genes. There is not necessarily a correlation between the tolerance of each growth and development period, and there are certain limitations in evaluating the stress tolerance of crops throughout the growth period only for the identification indicators of a certain development period.

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