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Method for extracting amino acids from mulberry leaf

A technology of amino acid and mulberry leaf, which is applied in the direction of fermentation, etc., can solve the problems of extraction of amino acid that have not been discovered, and achieve the effects of shortening the crushing time, increasing the extraction rate, and saving solvents

Inactive Publication Date: 2015-12-16
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, no patent has been found to extract amino acids from mulberry leaves by combined enzymatic hydrolysis and ultrasonic treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting amino acids from mulberry leaves, comprising the steps of:

[0037] (1) Pretreatment: picking fresh raw materials, air-drying, drying at 70°C, crushing, passing through a 20-mesh sieve to obtain mulberry leaf powder;

[0038] (2) Ultrasonic treatment: Take the mulberry leaf powder obtained in step (1) and add water or organic solvent according to the ratio of material to liquid ratio of 1g:15mL, and ultrasonically treat it for 45min at a power of 250W;

[0039] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:120mL, treat at 100rpm at 75°C for 35min, filter, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure ;

[0040] (4) Impurity removal: Add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:3, filter, and concentrate the filtrate to 1 / 5 of...

Embodiment 2

[0046] A method for extracting amino acids from mulberry leaves, comprising the steps of:

[0047] (1) Pretreatment: picking fresh raw materials, air-drying, drying at 70°C, crushing, passing through a 20-mesh sieve to obtain mulberry leaf powder;

[0048] (2) Ultrasonic treatment: Take the mulberry leaf powder obtained in step (1) and add water or an organic solvent at a ratio of 1g:10mL of material to liquid, and ultrasonically treat it for 40min at a power of 250W;

[0049] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:100mL, treat at 100rpm at 70°C for 30min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0050] (4) Impurity removal: add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:2, filter, and concentrate the filtrate to 1 / 5 of the original v...

Embodiment 3

[0055] A method for extracting amino acids from mulberry leaves, comprising the steps of:

[0056] (1) Pretreatment: picking fresh raw materials, air-drying, drying at 70°C, crushing, passing through a 10-mesh sieve to obtain mulberry leaf powder;

[0057] (2) Ultrasonic treatment: Take the mulberry leaf powder obtained in step (1) and add water or an organic solvent at a ratio of 1g:20mL of material to liquid, and ultrasonically treat it for 50min at a power of 250W;

[0058] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:150mL, treat at 100rpm at 80°C for 40min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0059] (4) Impurity removal: add absolute ethanol to the solution obtained in step (3), the volume ratio of the solution to absolute ethanol is 1:4, filter, and concentrate the filtrate to 1 / 5 of the original v...

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Abstract

The invention discloses a method for extracting amino acids from mulberry leaf, which uses mulberry leaf as the raw material and specifically comprises the following steps: (1) carrying out pretreatment; (2) carrying out enzymolysis and ultrasonic treatment; (3) carrying out decolorization; (4) carrying out impurity removal; (5) carrying out purification; and (6) dissolving the crystal, adding activated carbon, stirring, filtering, putting in a refrigerator, filtering, precipitating the amino acid crystal, and drying to obtain the composite amino acid crystal. Under the combined actions of the composite enzyme and ultrasonic on the mulberry leaf, the composite amino acids are extracted from the mulberry leaf; and the enzyme requires mild action conditions and has the wall breaking action, so that the amino acids in the mulberry leaf are thoroughly dissolved out. The ultrasonic extraction process is a physical process and does not have any chemical change; and in the extraction process, the extracted biological active substances are kept unchanged in a short time, thereby shortening the crushing time.

Description

technical field [0001] The invention relates to a method for extracting nutrients from mulberry leaves, in particular to a method for extracting amino acids from mulberry leaves. Background technique [0002] Mulberry leaf is a traditional Chinese medicine in my country, which contains a variety of functional components, and is also a kind of leaf protein with a relatively high content of Chinese plants. It was listed as a "medicine and food dual-purpose" plant by the Ministry of Health in 1993. my country is the largest mulberry tree planting country in the world, and mulberry leaves have great resource advantages in my country, and the market potential is huge. But since ancient times, the purpose of planting mulberry in our country is mostly for raising silkworms, and it is seldom processed and utilized in a deeper level. In recent years, in addition to exporting mulberry leaves to foreign countries every year, there is still a large amount of mulberry leaves in my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P13/04
Inventor 邓小玲宁成勇邓焱飞
Owner CHONGQING DOUHAO BIOTECH
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