A low-injury method for primary culture of colorectal cancer cells

Abstract

The invention relates to a low-injury primary culture method for colorectal cancer cells. The low-injury primary culture method comprises the following steps: (1) cell pretreating: treating colorectal cancer tissues with antibiotics; (2) dicing; (3) culturing: putting the diced tissue blocks into a culture medium containing the antibiotics for primary culture and normal culture till cell attachment is realized. The primary culture method has the advantages that the injury to the cells is low; the survival rate of the cells is high; the operation is simple; the culture is easy.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a low-damage primary culture method for colorectal cancer cells. Background technique [0002] Colorectal cancer is a common malignant tumor of the digestive system. In recent years, the incidence and mortality of colorectal cancer in my country have been increasing year by year. Most colorectal cancer patients are in the middle and advanced stages when they go to the doctor, and the treatment costs are high, the effect is poor, and the long-term survival rate is low. At the same time, it brings a heavy burden to patients, families and society. Colorectal cancer has obvious precancerous and early cancer lesions, and the time lasts for more than ten years. This provides opportunities for early detection and early treatment of colorectal cancer and precancerous lesions. The determination of tumor markers plays an important role in tumor diagnosis, curative effec...

AI Technical Summary

Problems solved by technology

[0006] The traditional enzymatic digestion method has the following defects: due to the digestion time (one sample can only select the same processing time, for example, the digestion time is set according to the standard of digesting a slightly larger tissue block into a single cell, which will inevitably result in smaller tissue Overdigestion of lumps and free cells), will cause uneven digestion of cells or even excessive digestion, damage cell membranes, destroy cells, affect cell activity, or incomplete digestion is not conducive to high-throughput screening experiments; there are many types of enzymes, in digestion The type and concentration of enzymes are variable when selecting enzymes, and different tissues have different requirements for enzymes, which requires complex conditions to explore

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