Molecular Breeding Method of Chestnut Varieties with Short Stamens
A short male inflorescence and molecular breeding technology, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of long selection period and low selection accuracy, and improve hybridization efficiency , the effect of reducing the amount of hybrid labor
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Embodiment 1
[0037] Example 1 Development of Molecular Markers Related to Chestnut Short Stamina Characters
[0038]Using the SLAF-seq (Specific-Locus Amplified Fragment Sequencing) technology developed by Beijing Biomike Biotechnology Co., Ltd. to carry out association analysis on the genetic segregation population of chestnut short male flowers, the molecular markers closely related to chestnut short male flowers were obtained. The specific process is as follows: use the chestnut genome to identify SNPs through bwa comparison software and GATK software. And filter the SNP. The filtering method is as follows: first filter the sites with a read support degree of less than 3, and then filter the sites in the offspring mixed pool that differ from the SNP types of the corresponding parents, and finally obtain high-quality credible SNP sites, and identify them on this basis The point of difference between the two pools. Finally, association analysis was carried out using the loci of differen...
Embodiment 4
[0064] Example 4 The method of cultivating new varieties of Chinese chestnut with insect resistance and storage resistance and short male inflorescence
[0065] The method comprises the steps of:
[0066] 1) Configure the hybrid combination: take the chestnut variety 'Heishanzhai No. 7' as the female parent, and select excellent germplasm resources such as 'Extra Early Maturity', 'Dayouli', 'Gushi No. 4' as the male parent, collect pollen and mix 'Heishanzhai 7' was pollinated. In the pollination process, 'Heishanzhai 7' did not need to be detasseled and bagged. The true hybrids were screened with primer I, and the F1 generation was obtained by hybridization.
[0067] 2) Constructing the gene haplotype molecular ID card of the hybrid parent: same as step 2) of Example 2.
[0068] 3) Molecular marker screening of hybrid seedlings: using the 'Gushi No. 4' haplotype molecular ID card and the molecular markers developed in Example 1 related to the chestnut short male inflorescenc...
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