Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation
A technology of seeds and Radix Paeoniae Rubra, applied in the field of promoting seedling emergence of Radix Paeoniae Rubra, can solve problems such as difficulty in meeting industrial production and market demand, failure to meet market demand, and low reproduction coefficient, so as to shorten emergence time and increase emergence rate , break the effect of dormancy
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Embodiment 1
[0020] Embodiment 1 (the embodiment under preferred parameter),
[0021] 1. Take the seeds of Radix Paeoniae Rubra and soak them in 0.5mM gibberellin solution at room temperature for 2 days, and the seeds are not moldy.
[0022] 2. After step 1 is completed, the seeds are taken and buried in moist vermiculite (with a water content of 8%±2%) at room temperature for 60 days.
[0023] 3, after completing step 2, get rooted and the seed that root length is more than 3cm, sow in the flowerpot (vermiculite is housed in the flowerpot, as culture medium; Sow 100 seeds in each flowerpot; Seed distance from vermiculite Surface 2.5 cm ± 0.5 cm), then divided into three groups, each with 3 pots, and treated as follows (the starting time of the grouping treatment is regarded as 0 time, and the number of days is counted from 0 time, and every 24 hours is 1 day):
[0024] The first group: cultured at room temperature;
[0025] The second group (prior art): first cultured at 4°C for 40 days...
Embodiment 2
[0032] Embodiment 2, exploration of relevant parameters
[0033] 1. Seed soaking time
[0034] Take the seeds of Radix Paeoniae Rubra and soak them with 1mM gibberellin solution at room temperature for 2 days or 5 days.
[0035] Three replicates were carried out, and 100 seeds were taken from each treatment for each replicate.
[0036] No mildew occurred in the seeds after 2 days of soaking. After soaking for 5 days, 8%±1% of the seeds were mildewed.
[0037] 2. Gibberellin concentration during seed soaking and burying time during burying
[0038] 1. Take the seeds of Radix Paeoniae Rubra and use different concentrations of gibberellin solution (the concentration of gibberellin is 0.001mM, 0.01mM, 0.1mM, 0.5mM, 1mM or 5mM, and the setting is to replace the gibberellin solution with 0.5mM calcium chloride solution The control CK) was soaked at room temperature for 2 days. None of the seeds were moldy.
[0039] 2. After step 1 is completed, the seeds are taken and buried i...
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