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Method for testing fecundity of holothuria scabra

A determination method and technology of rough sea cucumber, applied in the field of aquaculture, can solve the problems of invisibility, small eggs, certain difficulty in measuring the fecundity of rough sea cucumber, etc., and achieve the effect of simple and easy method and simple operation.

Inactive Publication Date: 2015-12-02
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the shape and structural characteristics of the ovary of rough sea cucumber are quite different from those of fish. The ovary is dendritic in shape and has a large number of reproductive branch tubules, which are dense and developed. The eggs are all wrapped in the branch tubules, and the eggs are not in a free state. In addition, There are also some immature eggs that adhere to the inner wall of the small tube and form dense clusters; and the eggs are small, with a diameter of 100-200um, only one tenth of that of fish eggs, and are almost invisible to the naked eye, so they can only be seen in count under microscope
However, it is difficult to measure the fecundity of sea cucumbers because the eggs are enclosed in the branching tubes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0018] ①Rough sea cucumber treatment: Take 1 head of fresh rough sea cucumber, wash the body surface with sea water, and then put it in the tray for 2 minutes to let the rough sea cucumber drain the water from the body.

[0019] ② Weighing and dissection: weigh the body weight (Wt) of rough sea cucumber (unit: g) on ​​an electronic balance with a sensitivity of 1 mg; dissect, extract all the ovaries with tweezers, and weigh the weight of the ovaries on an electronic balance (We) ( Unit: g); Then all the ovaries were soaked in a petri dish filled with saline.

[0020] ③ Sampling: Randomly take 2 branch tubes of the ovary from the petri dish, cut each small section with a length of about 5 cm with scissors, and weigh each small section on an electronic balance (denoted as W 1 , W 2 ) (unit: g), and the weighed branch segments were respectively soaked in 2 petri dishes filled with saline.

[0021] ④Break the wall: Take 2 small branch sections in turn, place them on a glass slid...

Embodiment

[0027] The method of the present invention will be described based on the examples of large mature female sea cucumbers and small immature female sea cucumbers collected respectively.

example 1

[0029] ①Treatment of rough sea cucumber: On April 2, 2015, 1 head of wild fresh rough sea cucumber was collected in Lingshui Bay, Hainan Province, transported back to the room, rinsed with sea water, and then placed in a tray for a few minutes to let the rough sea cucumber discharge moisture in the body.

[0030] ② Weighing and dissection: Weigh the rough sea cucumber on an electronic balance with a sensitivity of 1mg to 189.60g (Wt); dissect, extract all the ovaries with tweezers, and weigh the ovaries on an electronic balance to 26.49g (We) ; Then soak all the ovaries in a Petri dish filled with saline.

[0031] ③Sampling: Randomly take 2 segments of ovarian branch ducts, each cut out a small segment with a length of about 5 cm with dissecting scissors, and weigh each segment on an electronic balance. The weight of the first segment is 0.13g (W 1 ), the weight of the second small segment is 0.13g (W 2 ), and the weighed branch segments were respectively soaked in 2 petri d...

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PUM

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Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a method for testing the fecundity of holothuria scabra. The method comprises the implementation steps of holothuria scabra treating, weighing and dissecting, sampling, wall breaking and counting; fecundity calculating, wherein the absolute fecundity and relative fecundity of holothuria scabra are calculated according to the following formulas respectively: absolute fecundity=1 / 2*We*(N1 / W1+N2 / W2), and relative fecundity=1 / 2*We / Wt*(N1 / W1+N2 / W2). The simple method is utilized, a small branch tube wrapping eggs is broken through a dissecting needle, most of the eggs are released out of a tube cavity, and therefore the eggs can be counted under a microscope, and the fecundity of holothuria scabra is tested. The method is easy to operate, simple and feasible, serves development of artificial reproduction and production of holothuria scabra and can provide reference for testing the fecundity of sea cucumbers of other types.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, in particular to a simple and easy-to-operate method for measuring the fecundity of rough sea cucumbers. Background technique [0002] Fecundity refers to the fecundity of animals. The fecundity of aquatic animals is usually evaluated by the amount of fecundity of the individual, and there are two indicators of absolute fecundity and relative fecundity. Absolute fecundity is the total number of fecundity in an individual at a certain age, and relative fecundity is the ratio of the fecundity of an individual to its body weight. Determination of fecundity is very necessary in the artificial reproduction of aquatic animals. Before artificial reproduction, it is necessary to evaluate the gonad development status and maturity of the parents according to the fecundity, so as to select mature and sufficient parents, and choose the appropriate induction and breeding time , try to induce labor when t...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 杨学明江林源张立杨琼黄光华
Owner GUANGXI ACADEMY OF FISHERY SCI
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