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Method for promoting Anji white tea callus proliferation and improving tea polyphenol content in Anji white tea calluses

A technology of Anji white tea and callus, applied in the field of promoting callus proliferation and/or increasing the content of tea polyphenols in Anji white tea

Active Publication Date: 2015-12-02
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on how to promote the callus proliferation and / or increase the content of tea polyphenols in Anji white tea

Method used

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  • Method for promoting Anji white tea callus proliferation and improving tea polyphenol content in Anji white tea calluses
  • Method for promoting Anji white tea callus proliferation and improving tea polyphenol content in Anji white tea calluses
  • Method for promoting Anji white tea callus proliferation and improving tea polyphenol content in Anji white tea calluses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The induction of the callus of embodiment 1, Anji white tea

[0045] 1. Selection and disinfection of explants

[0046] Anji white tea was collected from Pingyuan Township, Dejiang County, Guizhou Province in May 2013, and it was confirmed according to the morphological and characteristic standards of Anji white tea cultivated in the original place, and the actual product was consistent with the name. Take the young Anji white tea leaves, wash them with tap water for 1 hour, disinfect them with 70% (volume fraction) ethanol for 30 seconds on the ultra-clean workbench, and then use 0.1% (mass fraction) HgCl 2 The solution was sterilized for 8 minutes, washed 5 times with sterile water, and then used.

[0047] 2. Induction of callus of Anji white tea

[0048] In the ultra-clean workbench, cut the leaves of Anji white tea sterilized in step 1 into pieces of about 0.5×0.5 cm in size, and inoculate them with different concentrations of 2,4-D, NAA, 6-BA, KT, Callus induction...

Embodiment 2

[0054] The proliferation of the callus of embodiment 2, Anji white tea

[0055] Select the good growth state callus obtained in Example 1, cut into small pieces with a scalpel on the ultra-clean workbench, inoculate in different callus proliferation medium, and inoculate 5 pieces per bottle (fresh weight is 0.8g, dry weight is 0.04g) explants, each treatment is 50 explants, repeats 3 times, harvests after 45 days. The culture conditions are temperature 25±2℃, light time 12h / day, light intensity 30~40mmol·m -2 ·s -1 .

[0056] The study found that the proliferation effect was the best when 0.5-4.0 mg / L TDZ and IAA were added to the medium, and the fresh weight and dry weight of the callus were 13.51 g and 0.83 g, which were 16.89 times and 16.89 times the initial inoculum amount. 20.75 times (Table 2). MS+TDZ0.5-4.0mg / L+IAA0.5-4.0mg / L is the best medium for callus proliferation. Callus proliferation medium (MS+TDZ0.5-4.0mg / L+IAA0.5-4.0mg / L) is specifically the medium obtai...

Embodiment 3

[0061] Embodiment 3, the effect of precursor substance on the proliferation of callus and the content of tea polyphenols

[0062] According to the result of embodiment 2, choose callus proliferation medium (MS+TDZ0.5mg / mL+IAA1.0mg / mL) as basic medium, add precursor substance therein, research precursor substance to callus Effects on growth and tea polyphenol content. In this embodiment, sodium acetate and phenylalanine are selected as precursor substances.

[0063] Select the good growth state callus obtained in Example 1, cut into small pieces with a scalpel on the ultra-clean workbench, inoculate in callus proliferation medium (MS) containing different concentrations of sodium acetate or phenylalanine +TDZ0.5mg / mL+IAA1.0mg / mL) (see Table 3 for the concentration of sodium acetate or phenylalanine), inoculate 5 pieces (fresh weight is 0.8g, dry weight is 0.04g) explant Each treatment was 50 explants, repeated 3 times, and harvested after 45 days. The fresh weight and dry wei...

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Abstract

The invention discloses a method for promoting Anji white tea callus proliferation and / or improving the tea polyphenol content in Anji white tea calluses. The method comprises the following step that Anji white tea calluses are placed in a culture medium A to be cultured, and therefore Anji white tea callus proliferation is promoted, and / or the tea polyphenol content in the Anji white tea calluses is improved. The culture medium A is obtained by adding TDZ, IAA and phenylalanine in an MS solid culture medium. The final concentration of the TDZ in the culture medium A is 0.5 mg / L, the final concentration of the IAA in the culture medium A is 1.0 mg / L, and the final concentration of the phenylalanine in the culture medium A is 0.1-1.0 mg / L. Experiments prove that, by adopting the culture medium, the ratio of fresh weight to dry weight and contrast of the Anji white tea calluses are significantly improved, and the tea polyphenol content in the Anji white tea calluses reaches 26.72% to the highest degree. The method is of great significance in development and utilization of Anji white tea resources and industrial production of tea polyphenol.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a method for promoting Anji white tea callus proliferation and / or increasing the content of tea polyphenols therein. Background technique [0002] Anji white tea is a famous Han tea produced in Xilong Township, Anji County, Zhejiang Province. It is an albino mutant variety that can be stably inherited in the tea (Camelliasinensis). Young leaves in spring are white, especially one bud and two leaves are the whitest, and the meridians are emerald green. As the temperature rises and the light increases, the leaf color gradually turns white, and the tea in summer and autumn is green. Anji white tea is originally produced in Anji County, Zhejiang Province. At present, Anji white tea has been introduced in Jiangsu, Jiangxi, Henan, Sichuan, Chongqing, Guizhou and other places. [0003] Anji white tea has good health effects. For example, Anji white tea contains a variety of amino ac...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王俊丽田璧瑞张一鸣黄婧婧张荣荣郭萌彭耀安卫东
Owner MINZU UNIVERSITY OF CHINA
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