A kind of cell cryopreservation solution and its application and cryopreservation method of megakaryotic progenitor cells
A technology of megakaryotic progenitor cells and cryopreservation methods, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of slow recovery of cell states and low cell viability, achieve good cell activity, maintain cell activity, damage reduction effect
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Embodiment 1
[0058] Prepare mother liquor:
[0059] Low-molecular-weight dextran mother liquor: purchased from Shanghai Changzheng Fumin Jinshan Pharmaceutical Co., Ltd., with a mass fraction of 10%.
[0060] Trehalose mother solution: fully dissolve 10mg trehalose powder in 0.5ml water
[0061] Human albumin mother solution: fully dissolve 120mg albumin in 3ml water
[0062] Add 0.5ml low-molecular dextran mother solution, 0.5ml trehalose mother solution, and 3ml human serum albumin mother solution to 5.5ml FBS in sequence, and finally add 0.5ml DMSO slowly to FBS, blow and mix gently, and store in a 4°C refrigerator .
Embodiment 2
[0064] Prepare mother liquor:
[0065] Low-molecular-weight dextran mother liquor: purchased from Shanghai Changzheng Fumin Jinshan Pharmaceutical Co., Ltd., with a mass fraction of 10%.
[0066] Trehalose mother solution: fully dissolve 10mg trehalose powder in 0.5ml water
[0067] Human albumin mother solution: fully dissolve 120mg albumin in 3ml water
[0068] Add 1ml low-molecular dextran mother solution, 0.5ml trehalose mother solution, and 3ml human serum albumin mother solution to 4.5ml FBS in sequence, and finally add 1ml DMSO slowly to FBS, mix gently by pipetting, and store in a 4°C refrigerator.
Embodiment 3
[0070] Prepare mother liquor:
[0071] Low-molecular-weight dextran mother liquor: purchased from Shanghai Changzheng Fumin Jinshan Pharmaceutical Co., Ltd., with a mass fraction of 10%.
[0072] Trehalose mother solution: fully dissolve 20mg trehalose powder in 1ml water
[0073] Human albumin mother solution: fully dissolve 80mg albumin in 2ml water
[0074] Add 1ml low-molecular dextran mother solution, 1ml trehalose mother solution, and 2ml human serum albumin mother solution to 5ml FBS in sequence, and finally add 1ml DMSO slowly to FBS, mix gently by pipetting, and store in a 4°C refrigerator.
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