Amylase mutants with improved thermal stability as well as coding gene and application of amylase mutants
A technology encoding gene and amylase, which is applied in application, genetic engineering, plant gene improvement, etc., can solve the problems of poor thermal stability and achieve excellent thermal stability
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[0025] After inserting two amino acids between the 208th and 209th amino acid residues of the α-amylase AMY121 in the thermostable bacterium Bacillussp.SCSIO15121 from deep sea vents, its thermal stability decreased significantly. Through homology modeling, it was found that the insertion of two amino acids significantly changed the conformation of Lys209. The amino acid sequence of the α-amylase AMY121 is shown in SEQ ID NO.1, and the nucleotide sequence of its coding gene—α-amylase gene amy121 has been deposited in Genbank, and its accession number is KJ577547.
[0026] 1 Identification of amino acid residue Lys209 related to thermostability
[0027] 1.1 Site-directed mutation of Lys at position 209 of α-amylase AMY121 mediated by inverse PCR
[0028] In order to further study the amino acid residue Lys209 related to the thermostability of α-amylase AMY121, starting from the α-amylase amy121 gene (KJ577547) of deep-sea hydrothermal vent bacteria Bacillussp. The primers wer...
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