Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Establishing method for rat central type superior mesenteric vein thrombosis model

A technique of venous thrombosis and method establishment, which is applied in the fields of medical science, veterinary instruments, veterinary surgery, etc., to achieve the effect of simple method and reduced experiment cost

Inactive Publication Date: 2015-11-18
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on making a model of superior mesenteric vein thrombosis using rats as model animals.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishing method for rat central type superior mesenteric vein thrombosis model
  • Establishing method for rat central type superior mesenteric vein thrombosis model
  • Establishing method for rat central type superior mesenteric vein thrombosis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but these embodiments do not constitute any limitation to the present invention.

[0028] The method for setting up the rat central type superior mesenteric vein thrombosis model provided by the present embodiment is carried out according to the following steps:

[0029] 1. Instruments and equipment:

[0030] (l) OPTON binocular surgical microscope (OPTON53, Germany);

[0031] (2) Microsurgical instruments;

[0032] (3) General surgical instruments;

[0033] (4) 7-0 atraumatic nylon suture.

[0034] 2. Establishment of central superior mesenteric vein thrombosis model:

[0035] (1) Selection of experimental animals:

[0036] Select healthy adult male SD rats (provided by Beijing Huafukang Biotechnology Co., Ltd.), weighing 250g-300g, and raise them in a ventilated, clean environment at 20°C-25°C for one week, free to eat and drink;

[0037] (2) Take...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an establishing method for a rat central type superior mesenteric vein thrombosis model. The model is established by establishing an acute central type superior mesenteric vein thrombosis model, ligaturing the trunk root of a superior mesenteric vein at a time through a 7-0 no-damage nylon suture at the position 0.2 cm below the joint of the free superior mesenteric vein and a splenic vein, and conducting abdomen closing. A sub-acute central type superior mesenteric vein thrombosis model is established by placing a metal stick with the diameter of 1 mm and a superior mesenteric vein in parallel at the position 0.2 cm below the joint of the trunk of the free superior mesenteric vein and the splenic vein, ligaturing the superior mesenteric vein and the metal stick together through a 7-0 no-damage nylon suture, retreating the metal stick after ligation so that opened calibers of all rats can be kept basically consistent, and conducting abdomen closing. Food and water are freely fed in the next day. Abdomen opening is conducted again 48 hours later, the superior mesenteric vein is completely ligatured, and abdomen closing is conducted. Food and water are freely fed in the next day after the second operation. The rat central type superior mesenteric vein thrombosis model is suitable for superior mesenteric vein thrombosis intestinal pathology and pathophysiology research.

Description

technical field [0001] The invention relates to a method for making an animal thrombosis model, in particular to a method for establishing a rat central superior mesenteric vein thrombosis model. Background technique [0002] There are many ways to make animal thrombus models, but they basically follow the classic principle of thrombus formation proposed by Virchow, that is, changes in the vessel wall, changes in blood properties, and changes in the state of blood flow. At present, the medical field has focused on blocking venous return, causing blood stasis to induce thrombus formation, supplemented by injecting thrombus-inducing agents or (and) mechanically damaging vascular endothelial cells, and established some relatively reliable and easy animal models. The specific methods are: 1. vein ligation (blocking) method; 2. mechanical injury to vascular intima; 3. electric current injury to vascular intima; 4. venous foreign body method; Thrombosis induced by thrombosis, etc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
Inventor 陈建立
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com