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Determination method of drought stress level of greenhouse crops

A technology for facility crops and drought stress, applied in the field of crop detection, to achieve high precision, accurate measurement, and relatively small error effects

Active Publication Date: 2015-11-18
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain limitations in studying the impact of drought on a certain index alone. At present, there are few previous studies on the comprehensive measurement method for determining the drought index of facility crops, and there are no suitable measurement methods.

Method used

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  • Determination method of drought stress level of greenhouse crops
  • Determination method of drought stress level of greenhouse crops
  • Determination method of drought stress level of greenhouse crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Step 1: Facility crop drought test

[0044] The crops in the seedling stage were transplanted into plastic pots. During the test, 4 water treatments were set. Normal irrigation T1 (70% to 80% of the maximum water holding capacity in the field) was the control group, and mild stress T2 (the maximum water holding capacity in the field 60% to 70% of the maximum field water capacity), moderate stress T3 (50% to 60% of the maximum field water capacity), severe stress T4 (field water capacity <50%), and three pots were set for each treatment.

[0045] The second step: equipment and medicine preparation

[0046] (1) Instruments and equipment

[0047] 10-1000μL, 1000-5000μL DRAGONLAB adjustable pipette gun, Shanghai Wandao Instrument Technology Co., Ltd.;

[0048] UV spectrophotometer, Shimadzu Corporation, Japan;

[0049] WP4-T dew point water potential meter, Beijing Ligaotai Technology Co., Ltd.;

[0050] Digital constant temperature water bath, Guohua Electric Co., Ltd....

Embodiment 2

[0096] According to the method of embodiment 1, utilize cauliflower to carry out drought test, set 4 water treatments, normal irrigation T 1 (70%-80% of the maximum water holding capacity in the field), as a control group, mild stress T 2 (60%-70% of the maximum water holding capacity in the field), moderate stress T 3 (50%-60% of the maximum water holding capacity in the field), severe stress T 4 (Field water capacity 1 was 48.5%, T2 was 32.4%, and T3 29.1%, T 4 was 21.6%. With the increase of the number of days after irrigation, the soil water content decreased slowly. On the third day after irrigation, the soil water content of each treatment tended to be stable and gradually approached. The soil water content of each treatment was: T 1 25.1%, T 2 21.0%, T 3 19.5%, t 4 During the test, the ambient temperature was 23°C / 17°C, the relative air humidity was 72%, and the photosynthetically active radiation was 800μmol·m -2 ·s -1 . Each treatment set up 3 pots to replica...

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Abstract

The invention discloses a determination method of a drought stress level of greenhouse crops. The method comprises the steps that drought treatments with different degrees are conducted on plants in the seedling stage, the leaf water potential, the root activity and the malondialdehyde content of the plants under the different treatments are determined, parameters under the different treatments are compared, a regression equation of crop drought stress indexes is established, the drought stress level is determined, and therefore the disaster situation is determined. According to the determination method of the drought stress level of the greenhouse crops, the root activity is determined through a TTC method, the MDA content is determined through a TBA reaction method, the leaf water potential is determined through a WP4-T dew-point water potential meter, the parameters are optimized and calculated by synthesizing physiological indexes of the crops, and the crop drought level is determined. By means of the determination method of the drought stress level of the greenhouse crops, the sizes of the the leaf water potential, the MDA content and the root activity are determined rapidly and accurately, the operational feasibility is high, the determination accuracy is high, the relative error is small, and the accurate calculation of greenhouse crop drought stress indexes and determination of the drought level can be met.

Description

technical field [0001] The invention belongs to the technical field of crop detection, and in particular relates to a method for determining the drought stress level of facility crops. Background technique [0002] Water is one of the most important substrates for photosynthesis of crops, and the level of water content directly affects the growth and development of crops. Only in a water environment suitable for crop growth can normal life activities be carried out. Insufficient or excessive water will have an impact on the physiology and morphology of crops. Studies have shown that when crops suffer from soil drought, the root system first feels the stress, which in turn affects the photosynthesis of leaves (Zuo Wenbo, 2010; Wang Lei, 2007). The reduction of photosynthesis under drought stress is limited by stomatal factors and non-stomatal factors. The first performance is stomatal closure (Ephrath, 1991). Stomatal closure inhibits the plant's response to CO 2 The absorp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00G01N33/00
CPCA01G7/00G01N33/0098
Inventor 杨再强侯奇奇
Owner NANJING UNIV OF INFORMATION SCI & TECH
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