A kind of primer for identifying Zoysia species and the identification method of Zoysia species

Abstract

The invention relates to a primer and method for recognizing zoysia japonica varieties. The nucleotide sequences of the primer are shown as the SEQ ID No.1 and the SEQ ID No.2. The invention further provides a method for recognizing the zoysia japonica varieties. The method comprises the steps that a genome DNA of a sample to be tested is used as a template DNA, and PCR amplification is carried out by means of the primer. Compared with an existing three-nature testing and recognizing method (namely the appearance, morphological characteristic description method) for recognizing zoysia japonica varieties, testing results are not affected by environment factors, operation is convenient and rapid, the testing period is obviously shortened, and the testing results are more accurate and reliable.

Description

technical field [0001] The invention belongs to the field of biological identification, and in particular relates to a primer for identifying zoysia varieties and a method for identifying zoysia varieties. Background technique [0002] China is the country with the richest zoysia resources in the world, and at the same time, zoysia is also the only turfgrass species whose seeds are exported in my country. The agricultural industry standard "Technical Regulations for the Approval of Grass Varieties" points out that the bred varieties should have distinctness, uniformity and stability (referred to as "DUS" or "DUS"). Therefore, before the approval of grass varieties, in addition to completing the uniformly arranged regional tests, new grass varieties should also pass the "three characteristics" test. At present, the method of "three characteristics" testing and identification of new grass varieties usually adopts the method of describing appearance and morphology. The test r...

AI Technical Summary

Problems solved by technology

Test results are easily affected by environmental factors, and the cycle is long

And with the increasingly narrow genetic basis of breeding materials and the increasing number of breeding varieties, the differences between varieties are getting smaller and smaller, and it is difficult to accurately identify them solely by traditional botanical characters.

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