Rapid Propagation Method of Uncaria macrophylla
A kind of fast technology, applied in the field of plant cultivation, can solve problems such as unreported, and achieve the effects of improving survival rate, improving survival rate, and improving tissue cell division.
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Embodiment 1
[0036] A rapid propagation method of Uncaria macrophylla, comprising the following steps:
[0037]Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut The 4cm-long tender stem segment with axillary buds is used as the explant of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, and then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 30 minutes, and then continue to rinse the explant with sterilized tea water 30s.
[0038] Step 2, transfer the sterile explants into the first culture medium...
Embodiment 2
[0049] A rapid propagation method of Uncaria macrophylla, comprising the following steps:
[0050] Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut The 2cm-long tender stem segments with axillary buds are used as explants of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar solution with a mass fraction of 1% for 25min, and then continue to rinse the explant with sterilized tea water 20s.
[0051] Step 2, transfer the sterile explants into the first culture medium...
Embodiment 3
[0062] A rapid propagation method of Uncaria macrophylla, comprising the following steps:
[0063] Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut A 3cm-long tender stem segment with axillary buds is used as an explant of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, and then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar solution with a mass fraction of 1% for 27 minutes, and then continue to rinse the explant with sterilized tea water 24s.
[0064] Step 2, transfer the sterile explants into the first culture...
PUM
![No PUM](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
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