Method for determining ammonium plundering capacity of a plant

A plant and ability technology, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problem of inability to quantitatively measure the absorption capacity of limiting ammonium nitrogen, and achieve the effect of fewer steps, simple calculation, and reliable data

Inactive Publication Date: 2015-11-04
INST OF GEOCHEM CHINESE ACADEMY OF SCI +1
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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a technical method for measuring the ammonium-grazing ability of plants, so as to overcome the deficiency that the prior art cannot quantitatively measure the absorbing ability of plants to limiting ammonium nitrogen

Method used

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  • Method for determining ammonium plundering capacity of a plant
  • Method for determining ammonium plundering capacity of a plant

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example

[0047] Example of the present invention: it comprises the following steps:

[0048] Firstly, the investigated plant and the reference plant Zhuge Cai were hydroponically cultured, and seedlings of the same size were selected, and the seedlings with the same number of plants were used as the unit, respectively, in the non-NH 4 + -N culture solution starvation culture 48h;

[0049] Second, put the investigated plants and the reference plant Zhuge Cai seedlings after starvation culture into the supplementary 0.2 mmol / L CaSO 4 Ammonium ion absorption experiments were carried out in the absorption solution containing ammonium ions.

[0050] Third, according to the above-mentioned ion absorption experiments, the Michaelis constants of the ammonium ion absorption of the investigated plant and the reference plant Zhuge Cai were respectively obtained. K m-ob and K m-o and the minimum concentration, that is, the external nutrient concentration when the net uptake rate of ammonium ...

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Abstract

The invention discloses a method for determining the ammonium plundering capacity of a plant. The method is characterized by comprising the steps that firstly, water culture is performed on the inspected plant and a reference plant orychophragmus violaceus; secondly, hungry culture is performed on the inspected plant and the reference plant orychophragmus violaceus in a culture solution without adding NH4<+>-N for 48 hours, and then an ammonium ion absorption experiment is performed; thirdly, michaelis constant and minimum concentration of ammonium ion absorption of the inspected plant and the reference plant orychophragmus violaceus are obtained according to the ammonium ion absorption experiment; fourthly, the ion concentration, namely the half inhibiting concentration, in the environment is further calculated when the absorption inhibition ratio of the inspected plant and the reference plant orychophragmus violaceus is 50 percent according to the michaelis constant and the minimum concentration; lastly, the ammonium plundering capacity of the inspected plant is calculated according to the half inhibiting concentration. The method is few in steps, simple in calculation and reliable in data; the ammonium plundering capacity of different plants in the same environment can be quickly and quantificationally obtained; technical support is supplied for screening Karst adaptable plants and excellent crop varieties.

Description

technical field [0001] The invention relates to a technical method for measuring the ability of plants to absorb limited ammonium nitrogen in a predatory manner, and belongs to the field of karst ecological management and agricultural engineering. Background technique [0002] China's karst (karst) distribution area is about 1.3 million Km 2 , of which the rocky desertification area accounts for about 28.7%. "South rock (rocky desertification) and northern sand (desertification)" have become two major ecological environmental problems that restrict the sustainable development of the western region. The bedrock in karst habitats is mostly carbonate rocks, the main chemical composition of which is CaCO 3 , MgCO 3 wait. Due to the long-term strong karstification, the hydrogeological structure in the karst area is a double spatial structure of surface and underground. The underground river system in this area is very developed, and there are many karst caves, karst depressi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 吴沿友吴沿胜赵红鹏张开艳杭红涛饶森陆叶姚凯刘莹
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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