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Application of circulating blood miR-34a in radiation monitoring as radiation biomarker

A biological marker, animal technology, applied in the field of radiation monitoring, can solve the problems of limitation, large irradiation dose, sensitivity and broadness, etc., to improve accuracy, reliability, stability and sensitivity. Effect

Inactive Publication Date: 2015-10-14
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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Problems solved by technology

It is worth noting that the radiation dose they choose is too large (1-8Gy), and it is limited to common electromagnetic radiation, and there are still great limitations in sensitivity and extensiveness when applied to radiation monitoring

Method used

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  • Application of circulating blood miR-34a in radiation monitoring as radiation biomarker
  • Application of circulating blood miR-34a in radiation monitoring as radiation biomarker
  • Application of circulating blood miR-34a in radiation monitoring as radiation biomarker

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Embodiment

[0028] Mice were irradiated with 0, 0.5, 2.0 and 4.0 Gy of X-rays, 6 mice per treatment group. Double-blind method for X-ray irradiation and circulating blood miR-34a expression level detection:

[0029] 1. Extraction of serum: after the radiation incident (6~72h is acceptable), collect the whole blood of the animal or human being to be identified into the EP tube without RNase, avoid violent shaking during the whole process, and do not let impurities fall into it tube to prevent hemolysis. At the same time, the whole blood of animals or humans not irradiated was collected as a control. Place the whole blood at room temperature for 1 hour, centrifuge at 3000 r / min for 10 minutes, absorb the upper clarified serum, put it into a new RNase-free tube, and centrifuge at 10000 r / min for 10 minutes to separate the serum needed for detection Samples can be stored at -80°C.

[0030] 2. Purification of RNA: Due to the low abundance of RNA in serum samples, it can be purified with the...

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Abstract

The invention relates to an application of circulating blood miR-34a in radiation monitoring as a radiation biomarker. The circulating blood miR-34a is extracted from animal or human serum through a routine method, and is used for judging the degree of damages of irradiation and radiation to animals or people when non-uniform irradiation or accidental irradiation is carried out for above 6h. The circulating blood miR-34a can be used to monitor the irradiation condition of people and animals in accidental radiation as a beneficial supplement of a physical dosimeter measurement result in order to effectively improve the assessment accuracy of radiation risks and radiation harms.

Description

technical field [0001] The invention relates to the technical field of radiation monitoring, in particular to the application of circulating blood miR-34a as a radiation biomarker in radiation monitoring. Background technique [0002] At present, radiation monitoring usually uses physical dosimeters, but in some cases, it is difficult to fully and accurately reflect the impact of radiation on each individual by simply using physical dosimeters for monitoring. For example, in the experimental research of animal irradiation, in order to reduce the chance of operators to be exposed to the radiation environment and improve the irradiation efficiency, automatic animal transfer devices are usually used. Sometimes the transported animals deviate from the irradiation center, resulting in non-uniform irradiation or even off-target irradiation. If the biomolecules carried by the animals themselves are used to confirm whether they are irradiated, it can not only reduce the waste of exp...

Claims

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Application Information

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IPC IPC(8): G01N33/49
Inventor 王菊芳危文俊王洁丁楠何进鹏
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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