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Myxobacteria strain and antitumor activity metabolite thereof

A metabolite, myxobacteria technology, used in antitumor drugs, bacteria, microorganisms, etc.

Active Publication Date: 2015-10-07
湖南晴天生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on anti-tumor active proteins in myxobacteria has not been reported yet.

Method used

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  • Myxobacteria strain and antitumor activity metabolite thereof
  • Myxobacteria strain and antitumor activity metabolite thereof
  • Myxobacteria strain and antitumor activity metabolite thereof

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Experimental program
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Effect test

Embodiment

[0033] ① Myxococcus STXZ72 of the present embodiment ( Myxococcus sp. STXZ72), the strain was preserved in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on June 4, 2015, and the strain preservation number is CCTCC NO: M2015353.

[0034] ② Myxococcus STXZ72 of the present embodiment ( Myxococcus sp.STXZ72) strain isolation process: collect the soil around cow dung at 29.73 north latitude and temperature range of 0-37°C, air-dry, grind, take 3 g of powder filtered through a 150-mesh sieve, add kanamycin 8 mg / mL; Ampicillin 30 mg / mL; ampicillin 30 mg / mL, 2 mL in total, cycloheximide (15 mg / mL) 1 mL, placed at 55°C for 10 min, and placed at room temperature overnight. The Escherichia coli grown to the logarithmic phase were collected and sterilized at 115°C for 30 min for later use. Add 20 μL concentrated E. coli to 20 μg / mL cycloheximide Wax medium. Drop the above-mentioned treated soil sample with a diameter of about 1 m...

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Abstract

The invention relates to a myxobacteria strain and an antitumor activity metabolite thereof. The myxobacteria strain is the myxococcus STXZ72, Myxococcus sp.STXZ72, and the preservation number of the strain is CCTCC NO:M2015353. The invention further comprises a separation and purification method of the antitumor activity metabolite of myxobacteria. The metabolite of Myxococcus sp.STXZ72 is prepared by utilizing the separation and purification method. Antitumor activity determination proves that the myxobacteria strain is capable of effectively inhibiting the activity of a variety of tumor cells and also has very low toxicity to normal human umbilical vein endothelial cells.

Description

technical field [0001] The present invention relates to a myxobacteria strain and its anti-tumor active protein, in particular to a high-efficiency anti-tumor wild-type strain ( Myxococcus sp.STXZ72) and its active hypothetical protein isolation and application. Background technique [0002] Myxobacteria are a class of Gram-negative unicellular rod-shaped, strictly aerobic chemoheterotrophic prokaryotic microorganisms. Dawid reported that myxobacteria are more likely to be distributed in low latitudes, slightly warmer, and milder climates. It can survive in the form of dormant body in the soil for more than several years, and can regerminate to form vegetative cells under suitable nutrient conditions for vegetative growth. Recent studies have shown that soil has become the main source material for the isolation of myxobacteria. [0003] Most of the currently known drugs such as antibiotics are basically derived from a small number of microorganisms, of which about 65% co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/52A61K38/48A61P35/00C12R1/01
Inventor 夏立秋文也丁学知
Owner 湖南晴天生物科技有限公司
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