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A kind of preparation method of Saccharomyces cerevisiae with high glutathione content

A glutathione content, Saccharomyces cerevisiae technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low glutathione content

Active Publication Date: 2018-01-23
保山九隆酵母有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can increase the intracellular glutathione content of Saccharomyces cerevisiae by more than 5 times, thereby further solving the problem of low glutathione content in microbial glutathione dry yeast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment one: Fermentation culture prepares high glutathione saccharomyces cerevisiae in shake flask

[0022] (1) Strains:

[0023] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in the 209 Laboratory of Bioengineering, Hubei University of Technology.

[0024] (2) Medium:

[0025] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;

[0026] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the seed medium is 5.8-6.2;

[0027] The fermentation medium per 1L of the shake flask level contains: glucose 60g, yeast powder 13g, ammonium sulfate 6g, potassium dihydrogen phosphate 2.5g, magnesium sulfate 0.3g, pH 5.5-6.0;

[0028] (3) Expanded cultivation of seeds:

[0029] After the slant seeds are activated for 12-16 hours, they are cultured in a triangular flask filled with seed medium, and the l...

Embodiment 2 0

[0038] Example 2: Saccharomyces cerevisiae with high glutathione content prepared by fed-batch fermentation on a 30L fermenter

[0039] (1) strain

[0040] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in the 209 Laboratory of Bioengineering, Hubei University of Technology.

[0041] (2) Medium

[0042] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;

[0043] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the seed medium is 5.8-6.2;

[0044]The bottom water in the fermentation tank horizontally contains: 9L of water, 270g of yeast powder, 22.5g of calcium chloride, 67.5g of magnesium sulfate pentahydrate, 0.072g of zinc sulfate, and 45g of potassium chloride. Natural pH. Add 4ml of defoamer. In addition: Add 0.45g vitamin B1, 0.45g vitamin B2, 13.5g vitamin B6, 0.45g nicotinic acid, 0.45g ca...

Embodiment 3

[0058] Embodiment three: Separation, washing and drying of high glutathione content Saccharomyces cerevisiae

[0059] (1) Isolation of Saccharomyces cerevisiae with high glutathione content

[0060] Pump the fermentation liquid into a vacuum drum with a vacuum degree of 0.08-0.1Mpa for separation, the drum speed is 50-80rpm per minute, and continuously separate the yeast cells. After the drum forms a filter cake, pump the filtrate into the drum for the second time , recovering the lost cells in the filtrate;

[0061] (2) Washing of Saccharomyces cerevisiae with high glutathione content

[0062] The washing described herein is to add 2-4 times the separated yeast cell volume temperature to the storage tank to disperse the yeast cells with tap water at room temperature, and then use vacuum drum separation under the same conditions as above, and repeat the washing for 2-3 times. Second-rate.

[0063] (3) Drying of Saccharomyces cerevisiae with high glutathione content

[0064...

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PUM

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Abstract

The invention relates to a preparation method of Saccharomyces cerevisiae with high content of glutathione. The method comprises the steps of activated culture, seed culture, fermentation, separation, washing and drying of Saccharomyces cerevisiae CICC1532. A Saccharomyces cerevisiae product with high content of glutathione can be obtained by optimizing culture conditions and feeding strategies and adopting relevant process means. The method provided by the invention can enable the intracellular content of glutathione in the Saccharomyces cerevisiae to be improved by more than five times, and thereby solves the problem that the content of glutathione in microbial glutathione dry yeast is low.

Description

technical field [0001] The invention relates to a method for preparing Saccharomyces cerevisiae with high glutathione content, in particular to a method for preparing Saccharomyces cerevisiae with high glutathione content by using agricultural and sideline resources. technical background [0002] Glutathione (GSH) has important physiological functions such as scavenging free radicals, detoxifying, inhibiting aging, preventing diabetes and cancer, inhibiting HIV, and improving human immunity; it has recently been found that glutathione can improve the immunity of animals and reduce the risk of antibiotics. The dosage has an important left and right. Therefore, Saccharomyces cerevisiae with high glutathione content has broad market prospects in the fields of medicine, food, cosmetics and animal protection. [0003] The production methods of glutathione mainly include extraction method, chemical synthesis method, enzymatic method and fermentation method. Among them, the ferme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/865
CPCC12N1/18
Inventor 蔡俊王常高杜馨
Owner 保山九隆酵母有限公司
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