Seed selection method for stichopus japonicas seeds with protosalt resistance
The technology of salt sea cucumber and seed is applied in the field of breeding of low-salt-tolerant sea cucumber seed, which can solve the problems of complex process, unstable low-salt tolerance of seed, etc. The effect of improving industrial output and economic benefits
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[0035] (1) On March 20, 2009, sea cucumbers that had been cultured for more than 1.5 years and had an individual weight of more than 300g / head were selected from a breeding pond in a certain area of the Yellow River Delta in Shandong as the basic population for breeding, and moved to industrialized breeding ponds separately. For temporary storage, the water in the temporary storage pool is mixed with natural seawater and underground well water to adjust the salinity to 20±1.5 and placed for 48 hours before use. Keep the pH value at 7.8-8.5, the dissolved oxygen above 5mg / L, and the temperature within 16°C. Feed the compound feed at 1% to 3% of the body weight of sea cucumbers every day, and stop 5 days before induced labor.
[0036] (2) On May 12, select individuals who still eat and grow normally and have strong vitality, and use the method of drying in the shade + running water stimulation to artificially induce labor. The egg-laying water temperature is 20°C and the salini...
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