Automatic passage type cell long-term experiment integrated module capable of being used for observing on line in real time

Abstract

The invention relates to a compact cell experiment module, namely an automatic passage type cell long-term experiment integrated module capable of being used for observing on line in real time, which not only has a function of updating an autonomous cell culture medium and a reagent and has an automatic passage ability to realize a long-term cell culture experiment, but also can be used for performing an individualized culture experiment and automatic sampling on cell samples of different culture rooms and can be used for observing the cell growth condition on line in real time. The automatic passage type cell long-term experiment integrated module comprises a reagent component, a culture medium component, a cell culture plate, a base, a multi-branch fluid driven flow path, an image system and a control system. The passage type cell long-term experiment integrated module provided by the invention not only has the function of updating the autonomous cell culture medium and the reagent and has the automatic passage ability of realizing the long-term cell culture experiment, but also can be used for performing the individualized culture experiment and automatic sampling on the cell samples of the different culture rooms and can be used for observing the cell growth condition on line in real time.

Description

technical field [0001] The invention relates to a cytology experiment module with long-term cell culture capability, especially integrated high-resolution online observation capability, and the reagents in the cell culture area can be automatically updated, sampled online, and stored reagents can be replenished in real time. A multifunctional cytology experiment module that can run autonomously. Background technique [0002] At present, in the field of cell biology, life science and related medical research, cytology experiments are often involved. Under laboratory conditions, cytology laboratories are often achieved by laboratory personnel manually manipulating cell culture flasks. Among the few automatic cell culture equipment on the market, a set of reagent replacement system is mostly used to serve multiple cell culture bottles or cell culture containers. The cell culture bottles are stacked and placed, and the reagent replacement system is installed in the stack of cel...

AI Technical Summary

Problems solved by technology

This structure can serve a large number of cell cultures / containers at the same time, but it also brings that all cell samples can only be used in a single culture and experiment mode, and cannot perform personalized experiment management or automatic sampling operations; especially the above-mentioned The cell culture of the model cannot realize the automatic passage of cells, and cannot meet the needs of long-term cell culture

At the same time, online real-time image observation cannot be realized for all cell samples, so it is difficult to monitor cell growth

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