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MTHFR gene polymorphism detection primer system and kit thereof

A gene polymorphism, detection kit technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of high price, low cost, cumbersome accuracy, etc., and achieve high sensitivity, wide application range, and wide application. field effect

Inactive Publication Date: 2015-09-09
上海赛安生物医药科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting MTHFR gene polymorphism mainly include chip technology, probe primer technology, enzyme digestion, pyrosequencing technology and HRM analysis technology, etc., which are expensive, cumbersome or not accurate.

Method used

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  • MTHFR gene polymorphism detection primer system and kit thereof
  • MTHFR gene polymorphism detection primer system and kit thereof
  • MTHFR gene polymorphism detection primer system and kit thereof

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Embodiment 1

[0034] 1. The composition of the kit.

[0035] The MTHFR gene polymorphism detection kit of this embodiment includes the reaction solution A for detecting the case where the c.1298 site of the MTHFR gene is A, and the reaction solution B for detecting the case where the c.1298 site of the MTHFR gene is C. Reaction solution C for detection of MTHFR gene c.677 site as C, reaction solution D for detection of MTHFR gene c.677 site as T, and reaction solution E for detection of β-actin gene as an internal control .

[0036] The preparation of reaction solution A, B, C, D, E is as follows:

[0037] 1. Preparation of reaction solution A (MIX A).

[0038] Reaction solution A is the reaction mixture for detecting the c.1298 position of the MTHFR gene, and the designed primers are aimed at the case where the c.1298 position of the MTHFR gene is A, including the forward arm for the case where the c.1298 position of the MTHFR gene is A Primer (Primer F A1298C For A), reverse primer (Pr...

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Abstract

The invention relates to an MTHFR gene polymorphism detection primer. The MTHFR gene polymorphism detection primer comprises a forward primer used when an MTHFR gene c.1298 locus is in an A condition, a reverse primer used when the MTHFR gene c.1298 locus is in the A condition, a forward primer used when the MTHFR gene c.1298 locus is in a C condition, a reverse primer used when the MTHFR gene c.1298 locus is in the C condition, a forward primer used when an MTHFR gene c.677 locus is in the C condition, a reverse primer used when the MTHFR gene c.677 locus is in the C condition, a forward primer used when an MTHFR gene c.677 locus is in the T condition, a reverse primer used when the MTHFR gene c.677 locus is in the T condition, a forward primer for detecting a beta-actin gene and a reverse primer for detecting the beta-actin gene. An MTHFR gene polymorphism detection kit is rapid and convenient to detect, high in sensitivity, high in accuracy rate and low in cost.

Description

technical field [0001] The invention relates to a gene mutation detection product, a detection primer and a detection system used in the product, and belongs to the field of biotechnology. Background technique [0002] Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the folic acid metabolism system, which can reduce 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, thereby serving as the methyl Indirect donors participate in the synthesis of purines and pyrimidines and the methylation of DNA, RNA, and proteins in the body, while maintaining normal homocysteine ​​levels in the body. MTHFR gene polymorphisms can lead to decreased activity of key enzymes in folic acid metabolism, resulting in folic acid metabolic disorders, leading to the occurrence of a variety of genetic diseases, including stroke caused by hyperhomocysteinemia and neonatal defects such as Down syndrome Syndrome (Down syndrome, DS) etc. are the most serious. MTHFR gene detection is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 赵新泰王明张长顺
Owner 上海赛安生物医药科技股份有限公司
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