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Tissue culture and propagation method for spring bamboo shoots

A technology of spring bamboo shoots and tissue culture, which is applied in the field of spring bamboo shoots tissue culture and reproduction, can solve the problems of seedling quality or too long cultivation growth cycle, increasing production cost and labor costs, hindering the development of seedling industrialization industry, etc., to shorten the production cycle and improve The effect of quality and root length

Inactive Publication Date: 2015-09-02
杨树东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spring bamboo shoot tissue culture seedlings have become a large number of seedling varieties, but because the production cost is increasing year by year, labor costs, site rent, seedling quality or too long cultivation growth cycle have greatly hindered the development of the seedling industrialization industry, so a kind of The new spring bamboo shoot tissue culture propagation technology overcomes the shortcomings of the original culture method and ensures the further development of spring bamboo shoot seedling industry

Method used

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  • Tissue culture and propagation method for spring bamboo shoots
  • Tissue culture and propagation method for spring bamboo shoots
  • Tissue culture and propagation method for spring bamboo shoots

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for tissue culture propagation of spring bamboo shoots, comprising the steps of:

[0037] (1) Selection of explants

[0038] It is 40cm long to choose, and the weight is 200g, and the spring bamboo shoots without damage by diseases and insect pests are explants;

[0039] (2) disinfection of explants

[0040] Wash the above-mentioned spring bamboo shoots with clear water and spray them with 75% alcohol for disinfection, then remove the shells, then use 75% alcohol to spray and disinfect the spring bamboo shoots, put them into a sterile ultra-clean table, and then use sterile water to clean them. Rinse the spring bamboo shoots, and then cut the bamboo shoots at 3 cm from the top of the spring bamboo shoots to ensure that the thickness of the cut bamboo shoots is 1 mm, then use 5% vitamin C aqueous solution to soak for 10 minutes, and then sterilize with 2% hypochlorous acid for 3 minutes. Then rinse 3 times with sterile water;

[0041] (3) Induced callus cultu...

Embodiment 2

[0056] A method for tissue culture propagation of spring bamboo shoots, comprising the steps of:

[0057] (1) Selection of explants

[0058] It is 50cm to choose long, and weight is 250g, and the spring bamboo shoots without damage by diseases and insect pests is explant;

[0059] (2) disinfection of explants

[0060] Wash the above-mentioned spring bamboo shoots with clear water and spray them with 75% alcohol for disinfection, then remove the shells, then use 75% alcohol to spray and disinfect the spring bamboo shoots, put them into a sterile ultra-clean table, and then use sterile water to clean them. Rinse the spring bamboo shoots, and then cut the bamboo shoots at 8 cm from the top of the spring bamboo shoots to ensure that the thickness of the cut bamboo shoots is 3 mm, then use 5% vitamin C aqueous solution to soak for 10 minutes, and then sterilize with 2% hypochlorous acid for 3 minutes. Then rinse 3 times with sterile water;

[0061] (3) Induced callus culture

...

Embodiment 3

[0076] A method for tissue culture propagation of spring bamboo shoots, comprising the steps of:

[0077] (1) Selection of explants

[0078] Choose long to be 45cm, and weight is 300g, and the bamboo shoots in spring without damage by diseases and insect pests is explant;

[0079] (2) disinfection of explants

[0080] Wash the above-mentioned spring bamboo shoots with clear water and spray them with 75% alcohol for disinfection, then remove the shells, then use 75% alcohol to spray and disinfect the spring bamboo shoots, put them into a sterile ultra-clean table, and then use sterile water to clean them. Rinse the spring bamboo shoots, and then cut the bamboo shoots at a distance of 4cm from the top of the spring bamboo shoots to ensure that the thickness of the cut bamboo shoots is 2.5mm, then use 5% vitamin C aqueous solution to soak for 10 minutes, and then sterilize with 2% hypochlorous acid for 3 minutes , and then washed 3 times with sterile water;

[0081] (3) Induce...

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Abstract

The invention discloses a tissue culture and propagation method for spring bamboo shoots. The tissue culture and propagation method is characterized by comprising the following steps: (1) explant selection; (2) disinfection treatment of the selected explants; (3) induction of callus culture; (4) differentiation culture; (5) strong seedling culture; (6) rooting culture; (7) acclimatization and transplanting. The tissue culture and propagation method provided by the invention has the advantages that not only can the productive culture single period and the growth cycle be shortened, and the occupied area for production be reduced, but also the survival rate of seedlings can be improved, so that the investment funds are reduced, the production cost is reduced, and the profits of industrialized production are increased.

Description

technical field [0001] The invention belongs to the technical field of tissue culture production, and relates to a method for tissue culture propagation of bamboo shoots in spring. Background technique [0002] Spring bamboo shoots are perennial evergreen herbaceous plants, and the edible parts are primary, tender, fat, short and strong buds or whips. Bamboo is native to China and has many types, strong adaptability and wide distribution. The underground stems of scattered bamboo species such as moso bamboo and early bamboo go deep into the soil, and the bamboo whips and shoots are protected by the soil layer, so they are not easy to be damaged by freezing in winter, and the shoots mainly come out in spring. Bamboo shoots are available all year round, but only spring and winter bamboo shoots taste the best. When cooking, no matter it is cold, fried or soup, it is fresh, tender and fragrant, and it is one of the delicacies that people like. [0003] The bamboo shoots dug a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨树东
Owner 杨树东
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