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Liver cirrhosis detection kit and detection method thereof

A technology for detection kits and detection reagents, which is applied in the biological field and can solve problems such as reliability discounts

Inactive Publication Date: 2015-08-26
TIANYIKANG TIANJIN HOSPITAL MANAGEMENT CO LTD
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Problems solved by technology

Among the ELISA detection methods, double-antibody sandwich ELISA is most commonly used. In the research of joint detection of serum peptides and proteins, this method generally detects several related or even unrelated protein or peptide markers separately, so that the reliability is greater. discount

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  • Liver cirrhosis detection kit and detection method thereof

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Embodiment Construction

[0032] Specific embodiments of the invention are described below:

[0033] A detection reagent for liver cirrhosis, comprising the following reagents: CHI3L1 microwell microtiter plate, CHI3L1 detection antibody, HRP-labeled avidin, HRP-labeled avidin diluent, CHI3L1 sample diluent, chromogen A, developer Colorant B, wash solution, stop solution, calibrators and quality controls.

[0034] The developer A is a buffer solution containing hydrogen peroxide, and the developer B is a buffer solution containing 3,3',5,5'-tetramethylbenzidine. The microwells of the CHI3L1 microwell plate are coated with rat anti-CHI3L1 monoclonal antibody, and the CHI3L1 microwell plate is provided with 8 microwells. The CHI3L1 detection antibody is a biotin-labeled goat anti-CHI3L1 polyclonal antibody. The stop solution is 1mol / L sulfuric acid solution.

[0035] The concentration of CHI3L1 in the calibrator is as follows: the concentration value is derived from LC-MS determination.

[0036]

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Abstract

This invention provides a liver cirrhosis detection kit and detection method, and belongs to the biotechnology field. According to the detection method, anti-human chitinase 3 protein 1 (CHI3L1) antibody coats a microporous plate for preparation of a solid phase antibody, when in use, a to-be-tested sample is added into the microporous plate coated with the antibody, if the to-be-tested sample contains a tested matter, the to-be-tested sample can be combined on the solid phase antibody to form an antigen-antibody complex; then a biotin-labeled anti-human CHI3L1 antibody is added to form an antibody-antigen-biotin-labeled antibody complex; horse radish peroxidase (HRP)-labeled avidin is added to form an antibody-antigen-biotin-labeled antibody-enzyme labeled avidin complex; and finally the complex is added into 3, 3 ', 5, 5 '-tetramethyl benzidine substrate system for chromogenic reaction. After the chromogenic reaction is completed, an enzyme standard instrument is used or testing. The liver cirrhosis detection kit has the advantages of fast, simple and accurate determination of test results.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for liver cirrhosis and a detection method thereof. Background technique [0002] The liver is the main site of protein synthesis in the human body. Various liver diseases can affect the protein synthesis function and cause changes in the concentration of various serum proteins. Liver cirrhosis is a common chronic, progressive, and diffuse liver disease caused by different etiologies. It produces diffuse hyperplasia of liver fibrous tissue on the basis of extensive deformation and necrosis of liver cells. Liver cirrhosis can cause synthetic dysfunction of liver cells. Reports on the probability of liver cirrhosis converting to liver cancer are not the same, and most studies still regard liver cirrhosis as the biggest risk factor for liver cancer. Therefore, early detection, early diagnosis, and early treatment of liver cirrhosis are very important. Interventional pre...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/085G01N2800/50
Inventor 欧阳玉岭
Owner TIANYIKANG TIANJIN HOSPITAL MANAGEMENT CO LTD
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