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Yulangsan tissue culturing method

A technology of tissue culture and Yulang Umbrella, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of large damage to explants and human body, residual disinfectant, poor disinfection effect, etc., to achieve thorough sterilization, Fast sterilization speed and good ventilation effect

Active Publication Date: 2015-08-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing disinfection treatments use hydrogen peroxide, sodium hypochlorite, antibiotics and mercuric chloride for disinfection, which have problems such as poor disinfection effect, residual disinfectant, great damage to explants and human body, and serious environmental pollution.

Method used

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  • Yulangsan tissue culturing method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A tissue culture rapid propagation method of Yulang umbrella, comprising:

[0038]Step (1), making Yulang umbrella explant: choose Yulang umbrella lateral buds, soak 5min with mass concentration successively as 1% detergent aqueous solution, rinse with tap water for 15min, rinse once with sterile water, then use volume concentration of Soak in 75% alcohol for 30s, then sterilize with 150 ml of 0.1% mercuric chloride added with 2-3 drops of Tween-20 for 6 minutes, rinse with sterile water for 3 times, and finally spread it on sterile filter paper to remove the surface water to obtain sterile explants.

[0039] Step (2), utilize the induction medium to cultivate the explant, obtain the test-tube plantlet after the explant germinates, the 6-benzylaminoadenine containing 1.5mg / L in the induction medium;

[0040] Step (3), utilize proliferation medium to cultivate test-tube plantlets, obtain test-tube plantlets to sprout in clusters, contain the kinetin of 0.5mg / L and the i...

Embodiment 2

[0053] A tissue culture rapid propagation method of Yulang umbrella, comprising:

[0054] Step (1), making Yulang umbrella explants: choose Yulang umbrella side buds, soak 10min with the mass fraction of 1% detergent solution successively, rinse with tap water for 30min, rinse once with sterile water, then use volume concentration of Soak in 75% alcohol for 50s, then sterilize with 150 ml of 0.1% mercuric chloride added with 2-3 drops of Tween-20 for 8 minutes, rinse with sterile water for 5 times, and finally spread it on sterile filter paper to remove surface moisture , to obtain sterile explants.

[0055] Step (2), utilize induction medium to cultivate explant, obtain test-tube plantlet after explant germination, contain the 6-benzylaminoadenine of 2.5mg / L in the induction medium;

[0056] Step (3), utilize proliferation medium to cultivate test-tube plantlets, obtain test-tube plantlets to sprout in clusters, contain the kinetin of 1.5mg / L, the 6-benzylaminoadenine of 0.5...

Embodiment 3

[0069] A tissue culture rapid propagation method of Yulang umbrella, comprising:

[0070] Step (1), making explants of Yulang umbrella: select the lateral buds of Yulang umbrella, soak them with a mass fraction of 1% detergent solution for 7 minutes, rinse with tap water for 22 minutes, rinse once with sterile water, and then use a volume concentration of 75 % alcohol for 40 seconds, then sterilized with 150 ml of 0.1% mercury liter added with 2-3 drops of Tween-20 for 7 minutes, rinsed with sterile water for 4 times, and finally spread it on sterile filter paper to remove surface moisture. Obtain sterile explants.

[0071] Step (2), utilize induction medium to cultivate explant, obtain test-tube plantlet after explant germinates, contain the 6-benzylaminoadenine of 2.0mg / L in the induction medium;

[0072] Step (3), utilize proliferation medium to cultivate test-tube plantlets, obtain test-tube plantlets to sprout in clusters, contain the kinetin of 1.0mg / L, the 6-benzylamin...

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Abstract

The invention discloses a Yulangsan tissue culturing method. The method comprises the following steps: induction culture, propagation culture, strong seedling culture and rooting culture, wherein the induction culture, the propagation culture, the strong seedling culture and the rooting culture are all performed in solid culture mediums. According to the method disclosed by the invention, a tissue culturing and quick propagating technology is adopted, so that good properties of original varieties are remained, the propagation coefficient of Yulangsan can be effectively and rapidly increased, the requirement for Yulangsan medicinal materials in the market can be met, and protecting the Yulangsan resources is also facilitated.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method of Yulang umbrella. Background technique [0002] Yulang Umbrella is a traditional folk medicinal material of the Zhuang nationality in Guangxi. It is mainly distributed in Guangxi, Guangdong and other places. It has the functions of nourishing qi, blood, and nourishing the lungs. Low back pain, rheumatic pain and chronic hepatitis and other diseases. After taking it, it can strengthen the body, eliminate fatigue, improve immunity and improve brain memory. [0003] At present, there are no reports on the breeding and cultivation of Yulang umbrellas. The folks mostly use wild resources. In recent years, with the continuous increase of market demand, the collection of wild resources has gradually increased, and the resources of wild Yulang umbrellas are on the verge of exhaustion. [0004] Most of the explants used for plant tissue culture are taken from the fiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 潘丽梅白隆华韦荣昌马小军冯世鑫黄芩芬
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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