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Automatic sampling system and control method of fermentation tank

A technology of automatic sampling and control methods, which is applied in the direction of biological material sampling methods, sterilization methods, and methods of supporting/immobilizing microorganisms, etc., which can solve the problems of small sampling volume, pollution, and the need for tens of days

Inactive Publication Date: 2015-08-19
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some fermentation processes take less than one day, while others require dozens of days
The method of sampling directly from the tank has a fast sampling speed and a small sample volume. However, for fermentations with a fermentation period of tens of days, although there is clean air in the sampling tube for isolation, there is still a certain risk of pollution, which needs to be carried out in the middle of fermentation. Sterilization of sampling lines

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  • Automatic sampling system and control method of fermentation tank
  • Automatic sampling system and control method of fermentation tank
  • Automatic sampling system and control method of fermentation tank

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Embodiment Construction

[0033] Attached below figure 1 , attached figure 2 , attached image 3 The present invention will be further described.

[0034]An automatic sampling system for a fermenter, comprising: a sampling device, connected to the fermenter 1 through a sampling valve 2, for taking out the fermented liquid from the fermenter 1; a sampling pool 14, which is internally equipped with a conductivity type for detecting liquid Liquid sensor 15; delivery pipe 9, one end of which is connected to sampling pool 14 through bidirectional sampling pump 16 through pipeline valve 21, and the other end is connected to sampling device through liquid sensor 10; waste liquid pipeline 17, one end of which passes through waste liquid valve 18 The two-way sampling pump 16 is connected to the sampling pool 14, and the other end is connected to the waste liquid bottle; and the sterile air pipeline 6 is connected to the delivery pipe 9 between the sampling device and the two-way sampling pump 16. Blow valve...

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Abstract

The invention discloses an automatic sampling system and a control method of a fermentation tank. In a sampling process, a sampling valve is controlled to be opened, a pipeline valve is controlled to be opened and a bidirectional sampling pump is controlled to be opened by a controller. When a fermentation liquor sample is detected by a liquid sensor, the controller starts timing and delays a certain time to control the sampling valve to be closed, and simultaneously controls a blowing pump and a blowing valve to be opened, and the sample in a delivery pipe is sent to a sampling pool by the sterile air through a sterile air pipe. When the fermentation liquor sample is detected by an electro-conductive liquid sensor in the sampling pool, the controller controls the bidirectional sampling pump to be closed and controls a waste solution valve to be opened, and the redundant fermentation liquor sample in the delivery pipe is discharged to a waste solution bottle through a waste solution pipeline. After the fermentation liquor sample is processed, the controller controls the waste solution valve to be opened and controls the bidirectional sampling pump to reversely rotate, and the fermentation liquor sample in the sampling pool is discharged to the waste solution bottle through the waste solution pipeline. The automatic sampling system is suitable for various fermentation sampling devices, various fermentation tanks can be automatically sampled, and thus an on-line analyzing system can be applied to various fermentation types.

Description

technical field [0001] The invention relates to an automatic sampling system and a control method of a fermentation tank. Background technique [0002] During the fermentation process, it is necessary to regularly sample and analyze from the fermenter to obtain various key data of the fermentation process. The most critical point in collecting fermentation samples from the fermenter is to ensure the safety of fermentation and prevent the contamination of the fermentation liquid by bacteria in the environment. Depending on the purpose of fermentation, the state of the fermentation product varies greatly. Some final products are very thin, some are very thick, and some are even agglomerated. Some fermentation processes take less than one day, while others require dozens of days. Fermentation tanks are also of various types and sizes. Large tanks have a capacity of hundreds of cubic meters, while small ones only have a few liters. For different fermentation types and fermen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/26C12M1/12C12M1/00
CPCC12M33/04C12M37/00C12M39/00C12M41/32
Inventor 朱思荣高广恒张金玲赵晓华张利群毕春元周万里
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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