Method for preserving plusia agnata staudinger eggs

A technology for Spodoptera californica and insect eggs, which is applied in the field of insect breeding, can solve the problems of inability to meet production needs, short refrigeration time for insect eggs, etc., and achieves the effects of improving production control ability and reducing the probability of rejuvenation.

Inactive Publication Date: 2015-08-12
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most important thing is that the egg storage time of the above-mentioned technology is too short to meet the production needs

Method used

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  • Method for preserving plusia agnata staudinger eggs
  • Method for preserving plusia agnata staudinger eggs
  • Method for preserving plusia agnata staudinger eggs

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Example 1: See the following series of comparative experiments.

[0012] In the comparison experiment, the pre-refrigeration process was as follows: take the eggs within 20 hours after production, put them in an airtight container, and fill them with quartz sand. Water with a mass ratio of 3%; fill the container with a mixture of nitrogen, oxygen, and carbon dioxide, and the volume mixing ratio of the three is 80:15:5, and the container is sealed.

[0013] The technology in the cold storage is: cool down the container to 2°C or 4°C at a rate of 4-5°C / h and refrigerate.

[0014] In the comparison experiment, the technical treatment in the recovery method is: raise the temperature of the above container to 7°C at a rate of 1.0°C / h, discard the stored gas, place it in the air, and continue to heat up to 11°C at a rate of 1°C / h. ℃, discard the quartz sand, continue to heat up to room temperature at a rate of 1 ℃ / h, and keep the humidity at 74% to 78%.

[0015] The followi...

example 2

[0018] Example 2: See the following experiment, which is a comparison of the effects of gas filling on egg preservation under the same conditions.

[0019] Take the eggs within 24 hours after production, put them in an airtight container, and fill them with quartz sand. The thickness of the quartz sand filling is 0.7mm, and the quartz sand contains 2% water by mass ratio; Fill it with different gases (see the table below for details), seal the container, cool the container to 4°C at a rate of 4-5°C / h, and refrigerate for 60 days.

[0020]

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Abstract

The invention provides a method for preserving plusia agnata staudinger eggs, and belongs to the technical field of insect breeding. Produced eggs within 24 hours are taken, placed into a closed container and are buried by silica sand, the container is filled with mixed gas of nitrogen, oxygen and carbon dioxide, and the container is closed. The temperature of the container is decreased to range from 2 DEGC to 4 DEG C at the speed ranging from 4 DEG C / h to 5 DEG C / h, and refrigerating is carried out. The temperature of the container is increased to range from 6 DEG C to 8 DEG C at the speed ranging from 8 DEG C / h to 1.2 DEG C / h, and preserving gas is removed and placed in the air. The temperature of the container is continuously increased to range from 10 DEG C to 12 DEG C at the speed ranging from 8 DEG C / h to 1.2 DEG C / h, and the silicon sand is removed. The temperature of the container is continuously increased to the normal temperature at the speed ranging from 8 DEG C / h to 1.2 DEG C / h, and the humidity is maintained to range from 70% to 80%, and normal hatching can be carried out. According to the method for preserving the plusia agnata staudinger eggs, under the low temperature ranging from 2 DEG C to 4 DEG C, the refrigerating time of the plusia agnata staudinger eggs can be increased to 120 days, the rejuvenation probability in the artificial breeding process is greatly decreased, the refrigerated eggs can be hatched according to needs, and the producing, regulating and controlling capacity is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of insect breeding. Background technique [0002] Silver leaf moth is a Lepidoptera Noctuidae insect that is widely harmful to Brassicaceae, Compositae, and Leguminous crops. At the same time, silver spider moth is also an economical insect with certain breeding value, but after many generations of artificial After breeding, the silver-leaved armyworm will show a tendency to decline in quality. When this happens, it is necessary to introduce wild populations for rejuvenation. Due to the short reproductive cycle of the silver-leaved armyworm, this rejuvenation process has become an unfavorable factor restricting large-scale production, and due to its potential economic value and the particularity of the feeding process, species conservation and on-demand hatching have become a technical problem. As early as 1994, Liu Jun et al. published "Research on Artificial Rearing Technology of Autographa", which stated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 高熹吴国星桂富荣田柏青赵维峰陈斌李成云李强李正跃
Owner YUNNAN AGRICULTURAL UNIVERSITY
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