High-resolution digital holographic microscopy imaging device and high-resolution digital holographic microscopy imaging method

Abstract

The invention discloses a high-resolution digital holographic microscopy imaging device and a high-resolution digital holographic microscopy imaging method. Based on a normal holographic device, the high-resolution digital holographic microscopy imaging device is improved by adding a distribution-known random phase plate between a small-hole diaphragm and an imaging device, so that more object light information can be scattered on a target surface of the imaging device after a sample to be tested is added. The high-resolution digital holographic microscopy imaging method includes the steps of collecting a group of holographic data, processing the holographic data by a filtering method to obtain distribution of object diffraction spots on the target surface of the imaging device, processing the diffraction spots by an improved iterative restoration algorithm similar to coherent diffractive imaging, and finally restoring a restructured image with the resolution rate much higher than that of a normal holographic image. The high-resolution digital holographic microscopy imaging device and the high-resolution digital holographic microscopy imaging method have the advantage that a solution for current low-resolution digital holographic microscopy imaging is provided.

Description

technical field [0001] The invention relates to digital holographic microscopic imaging, in particular to a high-resolution digital holographic microscopic imaging device and imaging method. Background technique [0002] In the field of holographic imaging, compared with traditional holography, digital holography has the characteristics of fast imaging speed and relatively low requirements for equipment stability. Therefore, it is widely used in the fields of microscopic imaging, three-dimensional imaging and particle tracking, and has achieved rapid development. . But at the same time, in the process of digital holographic recording and reproduction of object images, the imaging equipment (usually CCD) used to record holograms can achieve micron-level resolution, which is better than that of traditional holographic imaging using photosensitive plates to record. The low resolution of digital holography has become an important factor limiting its wide application. Scientist...

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Problems solved by technology

However, this method is difficult to deal with when there are defects such as dust on the surface of the grating, and the image interval of the holographic reconstruction is related to the period of the grating used. If the size of the imaging object is relatively large, it is easy to overlap the image, so it is only suitable for the size Imaging of sufficiently small objects, the scope of application is very limited

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