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A relative quantitative method for mass spectrometry of acute kidney injury biomarkers in urine

An acute kidney injury and biomarker technology, applied in the field of proteomics, can solve the problems of incomplete and definite accurate quantitative detection methods, and achieve simple and easy sample processing and quantitative methods, high throughput and good reproducibility. Effect

Active Publication Date: 2017-02-22
CUSABIO TECH LLC
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Problems solved by technology

[0008] At present, there is no complete and definite method for the accurate quantitative detection of tissue inhibitor of metalloproteinase (TIMP)-2 and insulin-like growth factor-binding protein 7 (IGFBP7). Diagnosis, to assist clinicians in the diagnosis of acute kidney injury, it is necessary to develop a method to accurately quantify these two substances, and provide a detection method for subsequent research and clinical application

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  • A relative quantitative method for mass spectrometry of acute kidney injury biomarkers in urine
  • A relative quantitative method for mass spectrometry of acute kidney injury biomarkers in urine

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Embodiment 1

[0030] Example 1 Tissue Inhibitor of Metalloproteinase (TIMP)-2 and Insulin-like Growth Factor Binding Protein 7 (IGFBP7) Biomass Spectrometry Relative Quantification Method in Urine Samples

[0031] For quantitative detection by mass spectrometry, it is required that the protein content of the sample tested on the machine is not less than 50ug, and the protein concentration is not lower than 5ug / uL. Therefore, pre-treatment is required for the collected urine samples. Proceed as follows:

[0032] (1) Sample collection and drying

[0033] Collect urine samples from normal people (i.e. the normal group) and patients with acute kidney injury (i.e. the patient group) for vacuum freeze-drying treatment, wherein the sample size of the normal group and the patient group are 3 samples, and each sample takes 200uL It is used for vacuum freeze-drying; the conditions for vacuum freeze-drying are: temperature -80°C, vacuuming by a compression pump.

[0034] (2) Sample reconstitution ...

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Abstract

The invention discloses a mass spectrometry relative quantification method for an acute kidney injury biological marker in urine. According to the technical scheme, a urine sample is subjected to enzymolysis to obtain multi-peptide fragment solution, liquid chromatography-tandem mass spectrometry is carried out on the multi-peptide fragment solution, then data is analyzed by adopting a biological informatical method, and thus relative quantification for a metalloprotease tissue inhibitor and an insulin-like growth factor binding protein 7 is realized. The mass spectrometry relative quantification method for the acute kidney injury biological marker in the urine has the characteristics of high flux, high sensitivity and good reproducibility.

Description

technical field [0001] The invention belongs to the technical field of proteomics, and in particular relates to a method for detecting biomarkers in urine of patients with acute kidney injury by using proteomics. Background technique [0002] Acute kidney injury (AKI) refers to abnormalities in kidney structure and function (including abnormalities in blood, urine, histology, and imaging examinations) within 3 months of the course of the disease. It is marked by a rapid decline in glomerular filtration rate. It is a clinical syndrome characterized by different etiologies and with different clinical manifestations. [0003] With the aging of the population, the incidence of diabetes, cardiovascular disease, and contrast nephropathy has increased, and the incidence of AKI has increased significantly in recent years. Despite the deepening understanding of AKI and the continuous progress of supportive and adjuvant treatment measures, the prognosis of AKI is still poor and the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 华权高沈鹤霄
Owner CUSABIO TECH LLC
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