Application of mussel meat oligosaccharides in the preparation of hypoglycemic drugs and its preparation method

A technology for hypoglycemic and diabetes drugs, which is applied to the application and preparation of clam meat oligosaccharides in the preparation of hypoglycemic drugs, and can solve the problems of weak biological activity, inability to treat early and effectively, and large molecular weight

Active Publication Date: 2017-12-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2008, the Diabetes Epidemiological Survey organized by the Diabetes Society (CDS) of the Chinese Medical Association showed that among people over 20 years old, the age-standardized prevalence of diabetes was 9.7%, and the proportion of prediabetes was as high as 12.8%. , which is equivalent to 1 out of 4 adults with hyperglycemia. What is more serious is that 60.7% of diabetic patients in my country have not been diagnosed and cannot be effectively treated early
At present, the molecular weight of mussel polysaccharides obtained by common extraction methods is generally relatively large, which is not easy to absorb and has weak biological activity, which limits the application of mussel polysaccharides in clinical medicine.

Method used

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  • Application of mussel meat oligosaccharides in the preparation of hypoglycemic drugs and its preparation method
  • Application of mussel meat oligosaccharides in the preparation of hypoglycemic drugs and its preparation method
  • Application of mussel meat oligosaccharides in the preparation of hypoglycemic drugs and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment 1 mussel meat oligosaccharide

[0012] Cut the mussel meat into pieces, add 95% ethanol to reflux twice, add 8 times the amount of 95% ethanol for the first time, and add 6 times the amount for the second time, each time for 1 hour, filter, discard the filtrate, and decoct the mussel meat with water Boil and extract twice, add 10 times the amount of water each time, decoct for 1 hour, filter, collect the filtrate and concentrate to a density above 1.2g / ml, add 95% ethanol to make the alcohol concentration reach 50%-60%, let stand More than 12 hours, filter, collect the filtrate and concentrate to a density of 1.2g / ml or more, add 95% ethanol again to make the alcohol concentration above 80%, let it stand for more than 12 hours, filter, collect the solid, and wash with absolute ethanol , Dry the precipitate at a low temperature of 50-60°C.

[0013] Dissolve the above-mentioned precipitate in an appropriate amount of distilled water, centrif...

Embodiment 2

[0014] Example 2 The hypoglycemic effect of mussel meat oligosaccharides on alloxan diabetic mice

[0015] 1. Animals

[0016] Kunming mice, SPF grade, male, provided by the Guangdong Medical Experimental Animal Center, animal qualification certificate number: SYXK (Guangdong) 2014-0020.

[0017] 2. Experimental materials

[0018] Mussel oligosaccharides: oligosaccharides extracted from the flesh of the clam clam, the oligosaccharides content of mussel oligosaccharides is higher than 93.5%, and the molecular weight ranges from 500-2000.

[0019] Mussel polysaccharides: polysaccharides extracted from the flesh of the clam clam, the clam polysaccharides contain more than 95.4% of polysaccharides, and the molecular weight ranges from 2500 to 15000.

[0020] 3. Experimental method

[0021] Take 90 male normal Kunming mice, after fasting for 24 hours, divide them into 2 groups randomly according to body weight, one group of 10 is the normal control group, and the remaining 80 ar...

Embodiment 3

[0030] Example 3 Hypoglycemic effect of mussel meat oligosaccharides on high-fat diet combined with streptozotocin-induced type Ⅱ diabetes rats

[0031] 1. Animals

[0032] SD rats, SPF grade, male, body weight 180-220g, provided by Guangdong Provincial Medical Experimental Animal Center, animal qualification certificate number: SCXK (Guangdong) 2014-0020.

[0033] 2. Experimental materials

[0034] Mussel oligosaccharides: oligosaccharides extracted from the flesh of the clam clam, the oligosaccharides content of mussel oligosaccharides is higher than 93.5%, and the molecular weight ranges from 500-2000.

[0035] Mussel oligosaccharides: oligosaccharides extracted from the flesh of the clam clam. The content of oligosaccharides in mussel oligosaccharides is higher than 95.4%, and the molecular weight ranges from 2500 to 15000.

[0036] 3. Experimental method

[0037]140 healthy SD rats were taken and fed with conventional diet for 7 days, and divided into normal control gr...

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Abstract

The invention relates to the application of mussel meat oligosaccharides in the preparation of blood sugar-lowering and anti-diabetic drugs or health food and its preparation method. The mussel meat oligosaccharide is preferably a mussel meat oligosaccharide compound with a molecular weight ranging from 500-2000, and its total content is higher than 50%. Experiments have proved that: mussel oligosaccharides can significantly inhibit the blood sugar level of alloxan-induced diabetic model mice and improve glucose tolerance; It has a significant hypoglycemic effect, mainly manifested in improving the abnormal glucose tolerance of type Ⅱ diabetic rats, inhibiting the increase of plasma HbA1c, significantly increasing the content of liver glycogen and muscle glycogen, strengthening the utilization of liver sugar and inhibiting the production of glycogen. Inhibit the secretion of glucagon, enhance the storage of glycogen and reduce the neogenesis of sugar; the acute toxicity test shows that mussel oligosaccharides have no toxic side effects and are highly safe.

Description

technical field [0001] The invention relates to a new therapeutic application of mussel meat oligosaccharides and a preparation method thereof. Background technique [0002] With the rapid development of the economy and the acceleration of industrialization, human health is facing an increasing threat from non-communicable diseases, and the prevalence of diabetes and the number of diabetic patients have risen sharply. Diabetes and its complications have brought serious burdens to human health and social development. According to the statistics of the International Diabetes Federation (IDF), there were 151 million diabetic patients in the world in 2000, and the current diabetic patients have reached 285 million. According to the current growth rate, it is estimated that nearly 500 million people worldwide will suffer from diabetes by 2030 . It is worth noting that diabetes is not only a "disease of the rich" in developed countries, but developing countries including China h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/702A61K31/715A61P3/10A23L33/00C07H3/06C07H1/08C08B37/00A23L33/125
Inventor 苏薇薇李沛波王永刚彭维吴忠
Owner SUN YAT SEN UNIV
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