Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polymyxin B production fermentation culture medium, preparation method and uses thereof

A fermentation medium and polymyxin technology, applied in the biological field, can solve the problem of low fermentation yield

Active Publication Date: 2015-07-15
SHANGHAI INST OF PHARMA IND CO LTD +1
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The relevant medium formulations involved in existing reports have the problem of low fermentation yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polymyxin B production fermentation culture medium, preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0037] Preparation method of fermentation medium

[0038] The culture medium provided by the invention is treated with amylase before sterilization.

[0039] Specifically, the preparation method of the fermentation medium provided by the invention comprises the following steps:

[0040] In the first step, the organic nitrogen source, whole wheat flour, inorganic salt and water are mixed according to the above formula to obtain a mixture 1;

[0041] In the second step, amylase is applied to the gelatinized or ungelatinized mixture 1 to obtain the fermentation medium provided by the present invention.

[0042] The amylase involved in the above method is selected from α-amylase, β-amylase, glucoamylase or isoamylase; preferred α-amylase; by the weight of the whole wheat flour dropped in the first step, the amylase The dosage is 2-1500U / g; preferably 2-1000U / g, more preferably 100-1000U / g; the action temperature of the amylase is 50-80°C, and the action time is 10-60 minutes; pr...

Embodiment 1

[0063] Culture strain: Paenibacillus polymyxa ATCC10401

[0064] Seed medium / %: corn steep liquor 2.0; glucose 5.0; ammonium sulfate 0.4; sodium chloride 0.1; calcium carbonate 0.3; pH6.8~7.0.

[0065] Fermentation medium / %: whole wheat flour 8.0; corn steep liquor powder 0.4; ammonium sulfate 0.9; potassium dihydrogen phosphate 0.03; calcium carbonate 0.03.

[0066] The fermentation medium was gelatinized, and BF7658α-amylase (Xingtai Wanda Bioengineering Co., Ltd.) was added. The amount of amylase was 100U / g, and the reaction was performed at 50°C-80°C for 30 minutes; after that, it was sterilized at 121°C for 20 minutes.

[0067] Cultivation method: inoculate the strains preserved on the slant into the seed medium, and cultivate at 28°C for 18 hours. Add 4% inoculum to 18L of the above-mentioned fermentation medium (the total volume of the fermenter is 35L), stir at 400r / min, under 50kPa tank pressure, ventilation at 1.2vvm, and culture at 30°C for 48h.

[0068] Determina...

Embodiment 2

[0071] Other operations are the same as in Example 1, except that the starting strain is NRRL B-719. After testing, the yield of polymyxin B obtained by cultivation was 3.14g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a polymyxin B production fermentation culture medium, a preparation method and uses thereof, wherein the culture medium comprises, by weight, 0.1-1.5 w / w% of an organic nitrogen source, 5.0-15.0 w / w% of whole wheat powder, and 0.3-1.2 w / w% of an inorganic salt.

Description

technical field [0001] The invention relates to the field of biotechnology; in particular, it relates to a fermentation medium and its preparation method and application. Background technique [0002] Polymyxin B (Polymyxin B, PMB) is a basic cyclic peptide antibiotic produced by Bacillus polymyxa or Paenibacillus polymyxa, which has good activity against Gram-negative bacteria. Colistin B drugs include polymyxin B sulfate injection and compound topical preparations of polymyxin B sulfate and other antibiotics, which are used to treat skin wounds, peritonitis, urinary system and respiratory system caused by Gram-negative bacteria Infection at other sites; PMB can also significantly inhibit the acute phase response induced by endotoxin, so it can also be used for sepsis. [0003] At present, the preparation of polymyxin B adopts the fermentation method. After the seeds of Bacillus polymyxa or Paenibacillus polymyxa are cultured, they are inoculated in a fermenter and cultiva...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/12C12R1/01
Inventor 马川川那可赵文杰魏晓东刘永双张守雨
Owner SHANGHAI INST OF PHARMA IND CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com