Massive preparation method of a high-precision 1/2 MS (Murashige & Skoog) culture substrate

Abstract

The invention discloses a massive preparation method of a high-precision 1 / 2 MS (Murashige & Skoog) culture substrate, and belongs to the technical field of plant biology. The method comprises the following steps: calculating 1000-times expanded quantity demand of 16 elements contained in the substrate and 10000-times expanded quantity demand of CuSO4.5H2O and CoCl2.6H2O; accurately weighting drug quantity; sequentially mixing the 1000-times expanded 16 elements according to the quantity demand from small to large; sealing; dissolving CuSO4.5H2O and CoCl2.6H2O with 1L of distilled water for later use; respectively opening to use as required. According to the method, the prepared substrate is high in precision; the method has the characteristics of being convenient and fast to operate, simple to apply, and small in error of different batches and the same batch, and is applicable to plant tissue culture and other biological applications.

Description

technical field [0001] The invention discloses a large-scale production method of a high-precision 1 / 2 MS culture substrate, belonging to the field of plant biotechnology. More specifically, it includes six steps including accurate calculation, accurate weighing, mixing less and more, sealing, separate mother liquor preparation, unpacking and using, in order to obtain different batches and batches with minimal error A method for cultivating substrates and reducing experimental errors. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the ...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters