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Method of identifying or evaluating beneficial actives and compositions containing same

A technology of active substances and tonicity, applied in the field of beneficial active ingredients, can solve the problems of not providing kinetic information, destructiveness, etc.

Active Publication Date: 2015-06-10
PROCTER & GAMBLE CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Additionally, both assays are destructive and do not provide kinetic information

Method used

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  • Method of identifying or evaluating beneficial actives and compositions containing same
  • Method of identifying or evaluating beneficial actives and compositions containing same
  • Method of identifying or evaluating beneficial actives and compositions containing same

Examples

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example 1

[0083] The following examples illustrate how the methods herein can be used to identify skin care actives. Hydrogen peroxide is a well-known ROS and was used in this example to demonstrate an undesired metabolic effect associated with exposure of cells to oxidative stress stimuli. Hydrogen peroxide was prepared at 10X the working concentration in the fibroblast assay medium described above. The agent tested was niacinamide (also known as vitamin B 3 ), which is commercially available from Sigma. Test agents were prepared at 10X the final working concentration in the fibroblast assay medium described above to provide test agent solutions. Both the stressor solution and the test agent solution were heated to 37°C and the pH was adjusted to 7.4. Cells were frozen human epidermal fibroblasts (BJ cell line) obtained from ATCC. Cells were cultured and prepared according to the assay methods described above. Fibroblasts were plated in 2 gelatin-coated 24-well V7 plates. Each we...

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Abstract

A method of identifying or evaluating skin-care actives that improve the metabolism of a skin cell. The method includes contacting cells with a stressor and a test agent and determining a response of the cells to the stressor and test agent, based on the change in metabolic indicators associated with glycolysis and / or oxidative phosphylation. The test agent may be identified as a skin-care active when the oxidative phosphorylation and / or glycolysis response corresponds to an improvement in cellular metabolism.

Description

technical field [0001] The present invention generally relates to methods of identifying or evaluating benefit active ingredients for use in personal care compositions. More specifically, the present invention relates to methods of identifying or evaluating active substances that counteract the effects of oxidative stress on skin cells. Background technique [0002] The fundamental basis of life is the need and ability of an organism to produce energy. In humans, food is ingested and converted into compounds such as adenosine triphosphate ("ATP") and nicotinamide adenine dinucleotide ("NAD"), which store the energy. Cellular metabolic pathways that convert useful components of food (eg, carbohydrates, fats, and proteins) into usable energy are complex and can be influenced by multiple factors in ways that are not yet fully understood. Mammalian skin cells are no exception. Skin cells are known to comprise many different types of cells that work together in a dynamic, com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5038A61K8/675A61K2800/10A61K2800/805G01N33/5044G01N2800/20
Inventor J·E·欧布龙H·A·罗维托
Owner PROCTER & GAMBLE CO
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