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A kind of method utilizing sisal waste water to ferment soybean meal

A technology of sisal wastewater and fermented soybean meal, which is applied in application, animal feed, animal feed, etc., to achieve the effects of high enzyme activity, shortened fermentation time, and increased bacterial content

Inactive Publication Date: 2017-12-15
建湖县高新投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention utilizes protease in sisal wastewater, lactic acid bacteria, and yeast to ferment and enzymatically hydrolyze soybean meal, greatly increasing the content of small peptides in fermented soybean meal. Promote the development of circular economy and environmental protection, and improve the product quality of fermented soybean meal, especially the content of small peptides is greatly improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Utilize the method for fermenting soybean meal with sisal waste water, its main steps are:

[0031] , Strain preparation

[0032] A, the preparation of lactic acid bacteria strain

[0033] Take out the preservation tube of lactic acid bacteria, use MRS solid medium to scratch the plate for recovery, and culture anaerobically at 37 degrees for 48 hours. Pick a single colony under the plate to inoculate 50 ml of MRS medium, and culture it anaerobically at 37 degrees in an incubator for 24 hours. The seeds are inoculated into the lactic acid bacteria seed medium in a 10L large triangular flask with an inoculum of 5%, cultured anaerobically at 37 degrees for 24 hours, and the concentration of the bacteria liquid is detected. The bacteria content is 6.5 billion / ml, which can be used as a lactic acid bacteria strain . The MRS medium described therein: peptone 10 g / L, yeast extract 5 g / L, beef extract 10 g / L, glucose 20 g / L, sodium acetate 5 g / L, diammonium citrate 2 g / L,...

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PUM

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Abstract

The invention relates to a method for fermenting soybean meal from sisal wastewater. To be specific, a waste by-product, namely, the sisal wastewater, of a sisal factory replaces externally-added water in the fermented soybean meal for soybean meal fermentation, special substances such as protease, organic acid and the like in the sisal wastewater are utilized, and the degradation rate of anti-nutritional factors in the soybean meal is greatly increased while the content of small peptides in the fermented soybean meal is increased. The method is simple in technology and low in cost, the problem of the sisal wastewater is solved for the sisal factory, the development of cyclic economy and environmental protection are promoted, the product quality of the fermented soybean meal is improved, and the content of the small peptides is greatly increased in particular.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for fermenting soybean meal with sisal leaf juice. Background technique [0002] As a plant protein raw material with stable quality, soybean meal is rich in amino acid types and content. Especially other plant feeds lack lysine, and its content is as high as 2.5%-2.8%. It is a high-quality fishmeal substitute plant protein. However, due to the existence of anti-nutritional factors (globulin antigen, phytic acid, tannin, urease, etc.) in soybean meal and most of the proteins are macromolecular proteins, the application value of soybean meal is limited. At present, the application of fermentation technology to process soybean meal is one of the effective ways to improve the quality of soybean meal. Fermented soybean meal refers to the use of beneficial microorganisms to ferment low-value soybean meal to remove various anti-nutritional factors, and at the same time produce a large amou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12
Inventor 李峰
Owner 建湖县高新投资发展有限公司
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