A kind of preparation method of composite photosynthetic bacteria preparation

A purple-sulfur photosynthetic bacteria, composite light technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problem of low survival rate, difficult to preserve, and no reports of the mixing of Rhodospirillaceae and Chromobiaceae. culture and other problems, to achieve the effects of rapid growth promotion, short lag period, and prolonged storage time

Active Publication Date: 2017-11-07
珠海鱼来鱼旺生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And up to the present, also do not see relevant technical proposal of the specific preparation method of Sulfur persicum to be disclosed
[0007] Moreover, from the existing literature, most of the composite photosynthetic bacteria only carry out mixed culture of bacteria in the family Rhodospiraceae, and there is no report on the mixed culture of bacteria in the family Rhodospiraceae and Chromobacteriaceae.
[0008] In addition, general photosynthetic bacteria are vegetative cells, which are not easy to store. Generally, the longest storage time at room temperature is 3-6 months. If the storage time is exceeded, the survival rate is very low. This is also a big disadvantage of photosynthetic bacteria in aquatic applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A kind of preparation method of compound photosynthetic bacteria preparation, adopts the Sulfur bacteria species in purple sulfur photosynthetic bacteria and the Rhodomicrobacter vannisi in the purple non-sulfur photosynthetic bacteria to cultivate according to the following steps:

[0047] 1. Activation and cultivation of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus tarotrichum, and culture it under light at 30°C for 8 days, and the bacterium thread to be punctured turns red and grows a lawn, that is It may be an activated Sulfur pericum species;

[0048] 2. Activation of Rhodomicrobium vannei on the flat plate: Streak the strain of Rhodomicrob vaninii on the plate for activation, activate and cultivate at 30°C for 4 days, and pick a large colony as the activated seed;

[0049] . Seed culture of Sulfur persicum: inoculate the activated strains into the liquid culture me...

Embodiment 2

[0058] A kind of preparation method of compound photosynthetic bacteria preparation, adopts the Sulfur bacteria species in purple sulfur photosynthetic bacteria and the Rhodomicrobacter vannisi in the purple non-sulfur photosynthetic bacteria to cultivate according to the following steps:

[0059] 1. Activation and cultivation of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus tarotrichum, and culture it under light at 30°C for 10 days, the thread to be punctured turns red and grows a lawn, that is It may be an activated Sulfur pericum species;

[0060] 2. Activation of Rhodomicrobium vannei on the flat plate: Streak the strain of Rhodomicrob vaninii on the plate for activation, activate and cultivate at 30°C for 4 days, and pick a large colony as the activated seed;

[0061] 1. Cultivation of S. peach seed: inoculate the activated strain into the liquid culture medium of S. peach...

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Abstract

The invention provides a preparation method of a compound photosynthetic bacterial preparation which can be stored for a long time and can quickly degrade harmful substances such as ammoniacal nitrogen and hydrogen sulphide in aquatic water. Seed activated culture, seed culture and illumination culture by virtue of an anaerobic fermentation tank are carried out on thiocapsa roseoppersicia in purple sulphur photosynthetic bacteria and rhodomicrobium vannielii in purple non-sulphur photosynthetic bacteria, so that a high-concentration compound photosynthetic bacterial preparation of the thiocapsa roseoppersicia and rhodomicrobium vannielii is obtained; and the compound photosynthetic bacterial preparation can quickly degrade the ammoniacal nitrogen and hydrogen sulphide in the aquatic water in aquatic and marine environments, so that the ammoniacal nitrogen and hydrogen sulphide in the aquatic water are reduced or eliminated, and yield and / or quality of aquatic and marine animals are / is improved.

Description

technical field [0001] The invention belongs to the technical scope of a functional microbial preparation, and in particular relates to a preparation method of a composite photosynthetic bacteria preparation that can quickly degrade ammonia nitrogen, hydrogen sulfide and other harmful substances in aquaculture water with long storage time. Background technique [0002] Many aquatic and marine environments, such as fish farms, ponds, ponds, lakes, estuaries, and oceans contain one or more aerobic zones and one or more anaerobic zones in the water column. In the aerobic zone near the surface of the water column, air and wind introduce oxygen, while aerobic bacteria produce phosphate, carbon dioxide, and ammonia, among others. In the anaerobic zone near the bottom of the water column, anaerobic microorganisms tend to produce hydrogen sulfide, ammonia, and methane, among others. Accumulations of hydrogen sulfide and ammoniacal nitrogen in anaerobic zones and underlying sediment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
CPCC02F3/34C02F2101/101C02F2101/16C12N1/20
Inventor 贺中华张进王胜望汤学敏杨勇张松董秋芬章太卓
Owner 珠海鱼来鱼旺生物科技有限公司
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