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A straw decomposing bacterial agent and its preparation method and application

A technology of straw decomposing bacteria agent and bacillus powder, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of high cost and labor, and achieve growth inhibition, important economic value, and simple preparation method Effect

Active Publication Date: 2017-10-20
FUJIAN SANJU BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Returning straw to the field as organic fertilizer after composting fermentation is a method widely used at present. Chinese patents 201010228347.3, 201310666396.9, and 201310308892.7 all provide straw decomposing bacterial agents for composting fermentation, and straw composting fermentation is labor-intensive, Disadvantages of high cost

Method used

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  • A straw decomposing bacterial agent and its preparation method and application
  • A straw decomposing bacterial agent and its preparation method and application
  • A straw decomposing bacterial agent and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 bacillus subtilis powder

[0026] 1. Activate the Bacillus subtilis stored in the refrigerator at 4°C, scrape the colony with an inoculation loop and inoculate it into the nutrient liquid seed medium, and culture it in a shaker flask at 30°C and 180rpm for 2 days. The liquid seed medium formula (g / L) was glucose 20.0, peptone 16.7, NaCl 5.3, beef extract 5.3, pH 7.0.

[0027] 2. Inoculate the prepared liquid seed liquid into the solid-state fermentation medium at a ratio of 100mL / kg, stir evenly, and ferment at 30°C for 8 days. The solid-state fermentation medium formula (W%) is bran 93.97%, soybean meal 4.94%, MgSO 4 ·7H 2 O0.05%, NH 4 SO 4 0.05%, CaCO 3 0.99%, after adding 50% water by weight of compost, stir evenly, and sterilize at 121°C for 20min.

[0028] 3. After expanded cultivation in shake flasks and solid-state fermentation for 8 days, the number of effective viable bacteria of Bacillus subtilis reaches about 6.7 billion / ...

Embodiment 2

[0030] The preparation of embodiment 2 Trichoderma longibrachiata powder

[0031] 1. Activate Trichoderma longibrachiatum stored in a refrigerator at 4°C to prepare a spore suspension, inoculate 1 mL of Trichoderma longibrachia spore suspension into 200 mL of nutrient liquid seed medium, and culture in a shaker flask at 30°C and 180 rpm for 2 days . The formula of the nutrient liquid seed medium is potato glucose medium powder 33.3g / L, natural pH, sterilized at 121°C for 20min.

[0032] 2. Inoculate the prepared liquid seeds into the solid-state fermentation medium at a ratio of 100 mL / kg, stir evenly, and ferment at a constant temperature of 30° C. for 8 days. The solid-state fermentation medium formula (W%) is bran 93.97%, soybean meal 4.94%, MgSO 4 ·7H 2 O0.05%, NH 4 SO 4 0.05%, CaCO 3 0.99%, add 50% water of compost weight, stir evenly, and sterilize at 121°C for 1h.

[0033] 3. Trichoderma longibrachiata was expanded and cultured in shake flasks, and after 8 days o...

Embodiment 3

[0035] Embodiment 3 Preparation of Streptomyces grisea powder

[0036] 1. Activate the Streptomyces grisea stored in a refrigerator at 4°C, scrape the spores of Streptomyces grisea with an inoculation loop and inoculate them in 200 mL of nutrient liquid seed solution shaker flask medium, and culture at 50°C and 180 rpm for 2 days. The liquid seed medium formula (g / L) of Streptomyces grisea is millet 20.0, KNO 3 1.0,K 2 HPO 4 0.5, MgSO 4 ·7H 2 O 0.5, NaCl 0.5, pH 7.2.

[0037] 2. Inoculate the prepared liquid seed solution into the solid-state fermentation medium at a ratio of 100mL / kg, stir evenly, cover the shallow plate (with a gap of 1cm), and ferment at 50°C for 5 days. Solid fermentation medium formula (W%) is millet 99.42%, KNO 3 0.1%, K 2 HPO 4 0.05%, MgSO 4 ·7H 2 O 0.05%, NaCl 0.05%, CaCO 3 0.33%, pH 7.2, millet is soaked in water at a ratio of 1.8mL / g for 24 hours, then sterilized at 121°C for 20 minutes, and then mixed with other ingredients.

[0038] 3. ...

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Abstract

The invention discloses a straw composting bacterium agent as well as a preparation method and application thereof and relates to the straw composting bacterium agent. The straw composting bacterium agent is compounded of 5-15 percent of bacillus subtilis powder, 50-70 percent of trichoderma longibrachiatum powder and 25-45 percent of streptomyces griseorubens powder. The preparation method comprises the following steps: respectively preparing zymophytes of bacillus subtilis, trichoderma longibrachiatum and streptomyces griseorubens and products thereof by adopting a solid fermentation method; drying and crushing to form the bacillus subtilis powder, the trichoderma longibrachiatum powder and the streptomyces griseorubens powder; mixing to obtain the straw composting bacterium agent. The straw composting bacterium agent can be applied to preparation of a straw composting and degrading agent.

Description

technical field [0001] The invention relates to a decomposing microbial agent, in particular to a straw decomposing microbial agent capable of rapidly decomposing crop straws in situ, a preparation method and application thereof. Background technique [0002] The main component of crop straw is fiber, and it is rich in nitrogen, phosphorus, potassium, calcium, magnesium and organic matter, etc. It is a renewable biological resource with multiple purposes. The average annual output of crop straw in my country is 700 million tons, one-third of which is discarded or incinerated, and straw incineration will increase CO in the air. 2 content and inhalable particulate matter, etc., not only cause waste of resources, but also lead to serious environmental pollution and endanger human health. [0003] At present, the main method to solve the large-area straw problem in my country is to return directly to the field, but the speed of returning to the field is slow and directly leads ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/125C12R1/885C12R1/465
CPCC05F11/08C12N1/14C12N1/20
Inventor 余劲聪林克明
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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