A kind of supramolecular hydrogel nanomaterial and gel factor precursor and its preparation method

A supramolecular hydrogel and nanomaterial technology, which is applied in the preparation methods of peptides, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of gel formation methods and gel properties. The research work has not been reported in the literature, etc. The problem is to achieve the effect of easy control of gel forming conditions, easy preparation and good water solubility.

Active Publication Date: 2017-07-21
WUXI DONGLIN TECH DEV
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Problems solved by technology

[0003] Naphthalene acetic acid-modified phosphorylated oligopeptide labeled with iodine atoms was used as the precursor of the gelling factor to form a supramolecular hydrogel nanomaterial through the action of alkaline phosphatase derived from bovine small intestine. Research work on gel properties has not been reported in the literature so far

Method used

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  • A kind of supramolecular hydrogel nanomaterial and gel factor precursor and its preparation method
  • A kind of supramolecular hydrogel nanomaterial and gel factor precursor and its preparation method
  • A kind of supramolecular hydrogel nanomaterial and gel factor precursor and its preparation method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0027] Embodiment 1: the synthesis of the first kind of pure compound P1 and the second kind of pure compound P2

[0028] Naphthaleneacetic acid-phenylalanine-4-iodophenylalanine-tyrosine-O-phosphate and naphthaleneacetic acid-phenylalanine-phenylalanine-tyrosine-O -Phosphate group Two NAA-modified phosphorylated oligopeptide fragments were synthesized separately.

[0029] The first pure compound P in this example 1 (Nap-Phe-Phe(I)-Tyr(PO(OH) 2 )) The synthetic route is as follows:

[0030] The NAA-modified phosphorylated oligopeptide sequence NAA-Phenylalanine-4-iodophenylalanine-Tyrosine-O-phosphate was synthesized by solid-phase peptide synthesis, according to: 1 mmol 2-chloro After trityl chloride resin was swelled in 2-3 ml of N,N-dimethylformamide for 4-8 minutes, 2 mmol of N-fluorenylmethoxycarbonyl-O-phospho-L-tyrosine was added, Then add 2 mmoles of N, N-diisopropylethylamine, react for 2-3 hours, react with 100 microliters of methanol for 5-10 minutes, and use 20...

Embodiment 2

[0040] Example 2: Testing experiment of mechanical properties of supramolecular hydrogel materials

[0041] Figure 7 It is the first pure compound P in this Example 2 1 The stress scanning spectrum of the storage modulus (G') and loss modulus (G") of the formed supramolecular hydrogel nanomaterial; Figure 8 It is the second pure compound P in this embodiment 2 2Stress scanning spectra of the storage modulus (G') and loss modulus (G") of the formed supramolecular hydrogel nanomaterials. Figure 9 It is the first pure compound P in this example 1 The dynamic frequency scanning spectrum of the storage modulus (G') and loss modulus (G") of the formed supramolecular hydrogel nanomaterial; Figure 10 It is the second pure compound P in this example 2 The dynamic frequency scanning spectrum of the storage modulus (G') and loss modulus (G") of the formed supramolecular hydrogel nanomaterials.

[0042] Depend on Figure 7 As can be seen from the square curve e of the storage m...

Embodiment 3

[0043] Example 3: Activity detection experiment of alkaline phosphatase derived from bovine small intestine

[0044] The first pure compound P with a concentration of 5.9 mmol per liter was used in the activity detection experiment of this embodiment. 1 , in a concentration of 10 mmol per liter of tris(hydroxymethyl)aminomethane, 50 mmol per liter of potassium chloride, 1 mmol per liter of magnesium chloride, 0.1 mmol per liter of zinc chloride and 200 In a solution with a unit volume of 10 microliters of alkaline phosphatase derived from bovine small intestine and a total volume of 100 microliters, incubate at 37 degrees Celsius for 10 minutes to complete the recognition and cleavage of the enzyme.

[0045] Figure 11 It is the first pure compound P prepared by the method of the present invention 1 The identification of alkaline phosphatase derived from bovine small intestine in vitro and the characterization results of high performance liquid chromatography tracking detect...

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Abstract

The invention discloses an iodine atom-labelled supramolecular hydrogel nanomaterial and an enzyme-sensitive hydrogel factor precursor and a preparation method of the hydrogel factor precursor. The iodine atom-labelled supramolecular hydrogel nanomaterial is characterized in that the iodine atom-labelled hydrogel factor precursor is obtained by synthesis reaction of solid-phase polypeptide, gel forming can be controlled by adopting a manner of adding alkaline phosphatase for constant-temperature incubation, and the activity of bovine small intestine-originated alkaline phosphatase can be intuitively displayed. Compared with the traditional supramolecular hydrogel nanomaterial, the iodine atom-labelled supramolecular hydrogel material has the advantages that the hydrogel factor synthesis is simple, and the gel forming conditions are easily controlled; the hydrogel factor precursor is labelled with iodine atoms which can effectively absorb X rays as heavy atoms, and the absorption of X rays at the gel forming position is more obvious compared with the absorption of other parts, so that the display on the distribution area of the alkaline phosphatase in different systems can be adapted. The iodine atom-labelled supramolecular hydrogel material and the enzyme-sensitive hydrogel factor precursor and the preparation method of the hydrogel factor precursor fill a blank of the heavy atom-labelled supramolecular hydrogel material and an enzyme-sensitive hydrogel factor precursor and a preparation method thereof.

Description

technical field [0001] The invention belongs to the technical field of supramolecular hydrogel nanomaterials, in particular to iodine atom-labeled supramolecular hydrogel nanomaterials, enzyme-sensitive gel factor precursors and preparation methods thereof. Background technique [0002] The magazine "Advanced Materials" (Adv. Mater., 2006, 18, 1365-1370) of the German Wiley Group reported supramolecular hydrogels with fluorenyl moxycarbonyl-protected oligopeptides as gelling factors. In this report, the gelling factor forms a supramolecular hydrogel by adjusting pH, that is, physical stimulation, which is not suitable for the application of biological systems. Supramolecular hydrogels using naphthalene acetic acid modified oligopeptides as gelling factors have been reported in the Journal of the American Chemical Society (J.Am.Chem.Soc., 2006, 128, 3038-3043), a journal of the American Chemical Society. reports. Compared with the fluorenylmethoxycarbonyl protecting group, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/087C07K1/06C07K1/04C12P21/02C12Q1/42
CPCY02P20/55
Inventor 梁高林唐安明
Owner WUXI DONGLIN TECH DEV
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