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Fermented cordyceps sinensis derived beta-glucan extract and preparation method thereof

A technology of fermenting Cordyceps and dextran, which is applied in food preparation, food science, application, etc., can solve the problem of loss of interest in fungal polysaccharides and achieve the effect of reducing the dosage

Active Publication Date: 2015-05-27
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because domestic research on Cordyceps polysaccharides is still at a relatively low level, some manufacturers even one-sidedly pursue the total polysaccharide content, adding maltodextrin, β-cyclodextrin and yellow dextrin, resulting in the loss of interest in fungal polysaccharides at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] a) Add ethanol with a mass concentration of 80% to the fermented wet Cordyceps mycelium, heat to 30° C., keep for 4 hours, separate in a decanter centrifuge at 3000 r / min, discard the filtrate, and store the wet mycelium for later use.

[0056] b) Add the wet mycelium in a), add purified water, the ratio of the bacteria to purified water is 1:3, stir for 5-30min, the speed is 10r / min-100r / min, and then decanter centrifuge at 3000r Centrifuge for 1 h under the condition of 1 / min, discard the filtrate, and obtain the mycelium after washing.

[0057] c) add purified water to the mycelium washed in b), the weight ratio of mycelium and purified water is 1:3, boil, keep warm for 6h, stir at 10r / min for 10min every 1h, and centrifuge Machine separation, discard the mycelium, retain the filtrate.

[0058] d) The filtrate obtained in c) is double-effect concentrated to a specific gravity of 0.8-1.3 at a vacuum degree of 0.02MPa-0.10MPa at 60°C, put into an alcohol sink, and add...

Embodiment 2

[0065] a) For the fermented wet Cordyceps mycelium, add ethanol with a mass concentration of 70%, heat to 40° C., keep for 2 hours, separate in a decanter centrifuge at 1000 r / min, discard the filtrate, and keep the wet mycelium for later use.

[0066] b) Add the medium wet mycelia in a), add purified water, the ratio of bacteria to purified water is 1:5, stir for 30min, the speed is 30r / min, and then centrifuge in a decanter centrifuge at 1000r / min for 1.5h , discard the filtrate to obtain the washed mycelium.

[0067] c) add purified water to the mycelium after washing in b), the weight ratio of mycelium to purified water is 1:5, boil, keep warm for 3h, stir at 10r / min for 5min every 1h, and centrifuge on a horizontal screw Machine separation, discard the mycelium, retain the filtrate.

[0068] d) Concentrate the filtrate obtained in c) with a vacuum degree of 0.02MPa-0.10MPa and double-effect at 40-50°C to a specific gravity of 0,8-1.3, put it into the alcohol settling pot...

Embodiment 3

[0074] The β-glucan extract prepared by the method in Example 1, sodium chloride and coffee powder were mixed with a three-dimensional motion mixer for 60-90 minutes according to the weight ratio of 1:0.05-0.30:10-30, that is, β-glucan sugar coffee.

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PUM

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Abstract

The invention discloses a fermented cordyceps sinensis derived beta-glucan extract and a preparation method thereof. The extract contains more than 50% of total polysaccharides, more than 40% of 1,3-1,6 beta glucan, and less than 10% of alpha glucan. The preparation method can be used for effectively extracting effective ingredients; a prepared product has concentrated beneficial ingredients of fermented cordyceps sinensis and can be used as a health product or drug, and the quantity of intaking can be obviously reduced.

Description

technical field [0001] The invention belongs to the application field of fungal resources, and relates to a preparation method of fermented cordyceps-derived β-glucan extract. Background technique [0002] β-glucan is a polysaccharide with multiple functional activities, the main chemical structure is β-1,3 glucan and β-1,6 glucan, the former has anti-tumor properties, and can greatly improve the human body natural immunity. [0003] The results of research by Japanese scientists have shown that β-glucan can regulate blood sugar levels and activate insulin activity, bringing good news to patients with hyperglycemia. Because domestic research on Cordyceps polysaccharides is still at a relatively low level, some manufacturers even one-sidedly pursue the total polysaccharide content and add maltodextrin, β-cyclodextrin and yellow dextrin, resulting in the loss of interest in fungal polysaccharides at home and abroad. [0004] In order to further develop and utilize fermented ...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/29A23L31/00
Inventor 王希艳徐国华黄盟盟张芙蓉王英燕李荀
Owner JIANGSU SHENHUA PHARMA
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