Large-scale manufacturing method of AA (containing carbon source and fixed medium) solid culture matrices

Abstract

The invention discloses a large-scale manufacturing method of AA (containing carbon source and fixed medium) solid culture matrices and belongs to the technical field of plant biology. The large-scale manufacturing method comprises the following steps: firstly calculating the demand quantity of 20 elements contained in the culture matrices after amplifying by 1000 times; accurately weighing the quantity of medicines after amplifying, wherein CuSO4.5H2O and CoCl2.6H2O need to be accurately weighed by using a millionth balance; sequentially mixing and sealing the 20 elements according to the demand quantity; and respectively unsealing and using according to needs. The AA (containing carbon source and fixed medium) solid culture media amplified by 1000 times can be prepared through the method, and the method has the characteristics of convenience in operation, high speed, simple application and small errors between different batches and in the same batch; and furthermore, the phenomena of great errors, precipitation, mildewing and the like caused by influence of manual operation and external factors are avoided during the preparation of AA liquid culture media, and the method can be applied to plant tissue culture and other biotechnical applications.

Description

technical field [0001] The invention discloses a large-scale production method of AA (containing carbon source and fixed medium) solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four cat...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters