Rapid identification of watermelon MLO type anti-powdery mildew gene

A powdery mildew resistance gene and watermelon technology, applied in genetic engineering, plant genetic improvement, plant peptides, etc., can solve the problems of difficulty in cloning and long time, and achieve the effect of shortening the excavation cycle, shortening the time, and improving the identification efficiency.

Inactive Publication Date: 2015-05-13
CHANGSHU CITY BEIBANG TOWN BEIGANG VEGETABLE SPECIALIZED COOP
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Problems solved by technology

However, because the basic research on watermelon is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of watermelon MLO type anti-powdery mildew gene
  • Rapid identification of watermelon MLO type anti-powdery mildew gene
  • Rapid identification of watermelon MLO type anti-powdery mildew gene

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Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the watermelon powdery mildew gene. The specific implementation process is as follows:

[0032] 1) Collection of watermelon MLO-type genes

[0033] In order to obtain all the MLO gene family members of watermelon, we first constructed an HMM model based on the MLO gene sequences of Arabidopsis thaliana and the powdery mildew resistance MLO gene sequences of tomato, pea, pepper, rose, pepper, and lotus root. Retrieve the MLO gene in different crops; secondly, use the published MLO gene sequence in different crops as the target sequence (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325904, TC3159 ,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383...

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Abstract

The present invention discloses rapid identification of watermelon anti-powdery mildew gene, relates to knowledge of plant comparative genomics, genetics, bioinformatics and other disciplines, and belongs to the plant biotechnology science field. The main steps of the invention comprise: 1) downloading the complete genome sequence of the watermelon, and collecting MLO type gene; 2) identifying the MLO type gene; 3) achieving the MLO type gene phylogeny relationship; and 4) comparing the MLO type powdery mildew gene. According to the invention, the watermelon powdery mildew gene finding period is effectively shortened so as to easily achieve rapid powdery mildew gene identification; the corresponding co-segregation function marker (SNP, SCAR and the like) is developed through the identified candidate powdery mildew gene, the watermelon anti-powdery mildew gene can further be rapidly used for the molecule marker-assisted selection of the anti-powdery mildew gene, and the accuracy is high; the watermelon anti-powdery mildew gene can be combined with other disease resistance gene molecule markers so as to create multi-resistance breeding materials, shorten the breeding period, and improve the breeding efficiency; and the foundation is established for exposition of the watermelon anti-powdery mildew molecule mechanism.

Description

technical field [0001] The present invention is to quickly identify watermelon powdery mildew genes by using methods such as plant comparative genomics, genetics, bioinformatics and candidate gene strategy by means of sequencing the whole genome sequence of watermelon, and mainly involves the download of the whole genome sequence of watermelon and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Watermelon (Citrullus lanatus) belongs to Cucurbitaceae, an annual vine herb. China is the largest producer of watermelons in the world, and diseases of watermelons include various diseases caused by fungi, bacteria and viruses. Powdery mildew is caused by an obligate living parasitic fungus, and it occurs widely in watermelon cultivation in protected fields and greenhouses. Spraying fungicides and planting disease-re...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G06F19/24C12N15/29C07K14/415
Inventor 钱孝英袁伟钱春桃
Owner CHANGSHU CITY BEIBANG TOWN BEIGANG VEGETABLE SPECIALIZED COOP
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