Large-scale production method for high-accuracy B5 culture medium

Abstract

The invention discloses a large-scale production method for a high-accuracy B5 culture medium, belonging to the field of plant biotechnology. The large-scale production method comprises the following steps: firstly, calculating the demanded quantities of 15 elements contained in the medium after 1000 times of amplification and the demanded quantities of CuSO4.5H2O and CoCl2.6H2O after 10000 times of amplification; accurately weighing the amplified medicine; sequentially mixing and sealing the 15 elements according to the demanded quantities respectively; dissolving CuSO4.5H2O and CoCl2.6H2O by 1L of distilled water for the later use; and unsealing as needed respectively. The high-accuracy B5 culture medium prepared by the method is convenient to operate, rapid, simple to apply, and has small errors of different batches and in the batches, avoids the phenomena of large errors, precipitation, mildewing and the like caused by the influences of manual operations and external factors, as well as can be applied to biotechnological application of plant tissue culture and the like.

Description

technical field [0001] The invention discloses a large-scale production method of a high-precision B5 culture substrate, belonging to the field of plant biotechnology. More specifically, it includes six steps including accurate calculation, accurate weighing, mixing less and more, sealing, separate mother liquor preparation, unpacking and using, in order to obtain different batches and batches with minimal error A method for cultivating substrates and reducing experimental errors. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters