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A kind of process method and process system for enzymatically synthesizing l-ascorbic acid 2-glucoside

A technology of ascorbic acid and process method, which is applied in the field of process method and process system for enzyme-catalyzed synthesis of L-ascorbic acid 2-glucoside, which can solve the problems of difficult removal of impurities, low yield, low purity of L-ascorbic acid 2-glucoside, etc. , to achieve the effect of stable and uniform crystal form, high product purity and less impurities

Active Publication Date: 2016-08-24
JIANGSU CHENGXIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing process for preparing L-ascorbic acid 2-glucoside, impurities are difficult to remove, the purity of L-ascorbic acid 2-glucoside is not high, and the yield is low

Method used

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  • A kind of process method and process system for enzymatically synthesizing l-ascorbic acid 2-glucoside

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Such as figure 1 , a process system for enzyme-catalyzed synthesis of L-ascorbic acid 2-glucoside, characterized by: comprising a reaction kettle 1, an enzyme storage tank 2, a storage tank 3, a centrifuge 4, a first filter device 5, and a first resin column 6 , the second resin column 7, the second filter device 8, the falling film concentration device 9, the third filter device 10, the discharge port 11; the first filter device 5 is located at the upstream of the first resin column 6, the The downstream of the first resin column 6 is the second resin column 7, the second filter device 8 is located downstream of the second resin column 7, and the falling film concentration device 9 is located at the second filter device 8 Downstream; the third filter device 10 is positioned at the downstream of the falling film concentration device 9; the centrifuge 4 has multiple groups, respectively upstream of the first filter device 5 and the third filter device 10 Upstream of and...

Embodiment 2

[0046]Add 20KG of drinking water to the 1000L reactor, then add 6.5KG of vitamin C, and stir; use hot water to control the internal temperature of the reactor to 37°C; use 30% NaOH solution to adjust the pH to 5.4; add β-cyclodextrin to the reactor 14KG, then add 4.0KG of CGT enzyme, add drinking water to make the system quantified to 65KG; control the temperature in the reactor at 37±2℃, and react in the dark for 10h; add 70g of glucoamylase (enzyme solution 750,000 U / ml), and control the temperature 37±2℃, avoid light for 10h.

[0047] After the reaction is completed, discharge the material, wash the reactor with 2.0KG of drinking water, combine the reaction solution and the water for washing the kettle, weigh, and centrifuge through a butterfly centrifuge, wash the centrifuge with 2.0KG of drinking water; pass the obtained filtrate through an ultra The filter is used for ultrafiltration. During the ultrafiltration process, 30.0KG of drinking water is added for ultrafiltrati...

Embodiment 3

[0050] Add 20KG of drinking water to the 1000L reactor, then add 6.5KG of vitamin C, and stir; use hot water to control the internal temperature of the reactor to 37°C; use 30% NaOH solution to adjust the pH to 5.4; add β-cyclodextrin to the reactor 14KG, then add 4.0KG of CGT enzyme, add drinking water to make the system quantified to 65KG; control the temperature in the reactor at 37±2℃, and react in the dark for 10h; add 70g of glucoamylase (enzyme solution 750,000 U / ml), and control the temperature 37±2°C, protected from light for 14 hours.

[0051] After the reaction is completed, discharge the material, wash the reactor with 2.0KG of drinking water, combine the reaction solution and the water for washing the kettle, weigh, and centrifuge through a butterfly centrifuge, wash the centrifuge with 2.0KG of drinking water; pass the obtained filtrate through an ultra The filter is used for ultrafiltration. During the ultrafiltration process, 30.0KG of drinking water is added f...

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Abstract

The invention provides a technological system and method for enzymatically synthesizing L-ascorbic acid 2-glucoside. The technological system comprises reaction kettles (1), an enzyme storage tank (2), a storage tank (3), centrifuges (4), a first filter device (5), a first resin column (6), a second resin column (7), a second filter device (8), a falling film concentration device (9) and a third filter device (10). The technological method comprises the steps of enzyme reaction, preparation of the crude product of L-ascorbic acid 2-glucoside and crude product refining. The technological system and the technological method have the technical effects that impurities are effectively removed, thus improving the purity of a finished product; the requirements for equipment are simple; the yield is high, after-treatment is simple to carry out, and the cost is low.

Description

technical field [0001] The invention relates to a process method and a process system for preparing L-ascorbic acid 2-glucoside, in particular to a process method and a process system for enzymatically synthesizing L-ascorbic acid 2-glucoside. Background technique [0002] L-Ascorbic acid 2-glucoside (also known as vitamin C glucoside, referred to as AA-2G, L-VCG), the chemical name is 2-oxo-α-D-glucopyranosyl ascorbic acid, which is a sugar derivative of vitamin C , has become the best substitute of vitamin C due to the following advantages: 1) good non-reducing activity, stable in aqueous solution; 2) good heat resistance and light resistance; 3) intracellular hydrolysis to produce vitamin C and glucose, Has the same biological activity as vitamin C. [0003] L-Ascorbic Acid 2-Glucoside is one of the 6 whitening additives approved by the Department of Health. It can directly discharge the melanin that has formed in the epidermis (that is, accelerate the reverse restorati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40C12M1/12C12P19/60C07H17/04C07H1/06
CPCC07H1/06C07H17/04C12M23/00C12M25/02C12P19/60
Inventor 邢将军祝俊许刘华任世阔吴锋余玉奎徐飞华俊国沈为标耿海义
Owner JIANGSU CHENGXIN PHARMA
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