Culture process of citric acid fermentation seed tank
A cultivation process and seed tank technology, which is applied in the direction of fermentation, microorganisms, fungi, etc., can solve the problems of unstable fermentation growth, unsatisfactory growth of strains, and uneven strains, so as to speed up the production intensity of fermentation and improve the stability of fermentation Sexuality, the effect of enhancing competitiveness
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Embodiment 1
[0026] A kind of citric acid fermentation seed tank cultivation technique, its concrete steps are:
[0027] (1) Put 20.50g / 100mL corn liquefaction solution of total sugar into the seed tank, add drinking water and 0.04% N source accounting for the total mass of the medium at the same time, heat up to 121°C for disinfection for 30 minutes, cool down to 29.5°C, The detected total sugar is 8.50g / 100mL; prepare Aspergillus niger spore suspension, according to the ratio of 790,000 spores inserted into each milliliter of seed liquid, count by hemocytometer, calculate the required volume of spore suspension, pass through the flame circle The spore suspension is inserted into the medium in the seed tank for cultivation;
[0028] (2) In the 0-12h stage of citric acid fermentation seed tank culture, control the culture temperature: 29.5-33.5 ° C; tank pressure: 0.06-0.08 MPa; ventilation ratio: 0.06-0.07 (V / V min); stirring rate: 55 -57rpm.
[0029] (3) In the 12-16h stage of citric a...
Embodiment 2
[0036] A kind of citric acid fermentation seed tank cultivation technique, its concrete steps are:
[0037] (1) Pour 23.50g / 100mL corn liquefaction solution into the seed tank, add drinking water and 0.07% N source of the total mass of the medium at the same time, heat up to 121°C for 30 minutes, and cool down to 28.5°C, The detected total sugar is 11.80g / 100mL; prepare Aspergillus niger spore suspension, according to the ratio of 830,000 spores inserted into each milliliter of seed liquid, count by hemocytometer, calculate the required volume of spore suspension, pass through the flame circle The spore suspension is inserted into the medium in the seed tank for cultivation;
[0038] (2) In the 0-12h stage of citric acid fermentation seed tank culture, control the culture temperature: 28.5-34.5 ° C; tank pressure: 0.06-0.095 MPa; ventilation ratio: 0.07-0.08 (V / V min); stirring rate: 53 -55rpm;
[0039] (3) In the 12-16h stage of citric acid fermentation seed tank culture, c...
Embodiment 3
[0046] A kind of citric acid fermentation seed tank cultivation technique, its concrete steps are:
[0047] (1) Put 21.60g / 100mL corn liquefaction solution of total sugar into the seed tank, add drinking water and 0.06% N source accounting for the total mass of the medium at the same time, heat up to 121°C for 30 minutes, and cool down to 29.8°C, The detected total sugar is 9.40g / 100mL; prepare Aspergillus niger spore suspension, according to the ratio of 740,000 spores inserted into each milliliter of seed liquid, count by hemocytometer, calculate the required volume of spore suspension, pass through the flame circle The spore suspension is inserted into the medium in the seed tank for cultivation;
[0048] (2) In the 0-12h stage of citric acid fermentation seed tank culture, control the culture temperature: 29.8-34.8°C; tank pressure: 0.065-0.085MPa; ventilation ratio: 0.06-0.07 (V / V min); stirring rate: 56 -58rpm;
[0049] (3) In the 12-16h stage of citric acid fermentati...
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