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A kind of method for extracting genistin from Dayejinjinba

A technology of genistein and genistein is applied in the field of extraction of natural products, and can solve the problem of no separation method for genistein, and achieve the effects of saving reagents, good reproducibility and simple operation.

Active Publication Date: 2016-06-22
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, patent document CN1876671 discloses a soybean isoflavone extract extracted from soybean meal and an extraction method, the extract contains genistin, daidzin and daidzein, and the product provided by the method is Mixture of isoflavone glycosides without separation method for genistin

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  • A kind of method for extracting genistin from Dayejinjinba
  • A kind of method for extracting genistin from Dayejinjinba

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Genistin was prepared as follows:

[0038] (1) Cut 20kg of Daye Qianjinba into 2cm-long fragments, and extract twice with 60% ethanol aqueous solution; for the first time, add 160L of ethanol aqueous solution to the raw material, reflux extraction for 2 hours, and filter to obtain the first time Extract and filter residue; add 120 L of ethanol aqueous solution to the filter residue, reflux for extraction for 2 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;

[0039] (2) Concentrate the extract obtained in step (1) to 5L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3 to 1.25mm in the stationary phase are not less than 95 %; Carry out pre-elution with water of 2 times of chromatographic column volume, abandon pre-eluent; Carry out elution with the ethanol-water ...

Embodiment 2

[0043] Genistin was prepared as follows:

[0044] (1) Cut 20kg of Daye Qianjin into 2cm fragments, and extract twice with 50% ethanol aqueous solution; for the first time, add 150L ethanol aqueous solution to the raw material, reflux extraction for 2.5 hours, and filter to obtain the first time Extract and filter residue; add 110 L of ethanol aqueous solution to the filter residue, reflux extraction for 2.5 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;

[0045] (2) Concentrate the extract obtained in step (1) to 7.5L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3-1.25mm in the stationary phase are not less than 95%; pre-elute with 2 times the volume of the chromatographic column, and discard the pre-eluent; then elute with ethanol-water solution with a volume ratio...

Embodiment 3

[0048] Genistin was prepared as follows:

[0049] (1) Cut 20kg of Daye Qianjinba into 3cm fragments, and extract twice with 70% ethanol aqueous solution; for the first time, add 170L of ethanol aqueous solution to the raw material, reflux extraction for 1.5 hours, and filter to obtain the first time Extract and filter residue; add 130 L of ethanol aqueous solution to the filter residue, reflux for extraction for 1.5 hours, and filter to obtain the second extract; combine the extracts obtained from the two extractions to obtain the extract;

[0050] (2) Concentrate the extract obtained in step (1) to 7L and put it on the chromatographic column. The stationary phase in the chromatographic column is D101 macroporous adsorption resin, and the resin particles with a particle size of 0.3-1.25mm in the stationary phase are not less than 95 %; Carry out pre-elution with the water of 1.5 times of chromatographic column volume, abandon pre-elution liquid; Carry out elution with the etha...

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Abstract

The invention relates to a method for extracting genistin from largeleaf flemingia. The method comprises the following steps: (1) chopping the raw material largeleaf flemingia and performing reflux extraction by use of ethanol water having the concentration of 50-70% to obtain an extract; (2) concentrating the extract obtained in the step (1) and putting on a chromatographic column, wherein the fixed phase in the chromatographic column is macroporous adsorption resin; eluting with an ethanol-water solution in the volume ratio of (15-25): (85-75) as a moving phase and collecting the eluate; and (3) concentrating the eluate obtained in the step (2) into a thick extract, adding ethanol containing 2-3% of glacial acetic acid for thorough dissolving, standing, filtering out insoluble substances, recrystallizing the filtrate, washing crystals by use of ethanol, and drying in vacuum to obtain the genistin. The method has the advantages of rich and easily available raw materials, simple and feasible process, low pollution and short period; and the obtained product is high in purity and prone to large-scale preparation.

Description

technical field [0001] The present invention relates to the extraction of natural products, in particular to a method for extracting genistin from Echinacea japonicus. Background technique [0002] Genistin is a kind of isoflavone compound, which has estrogen-like effect in metabolism in the body, and is called phytoestrogens. Genistin is hydrolyzed into genistein under the action of human intestinal flora, and plays a role by competitively binding to estrogen receptors. It is mainly used in the pharmaceutical industry to treat gynecological diseases. [0003] In the prior art, patent document CN1876671 discloses a soybean isoflavone extract extracted from soybean meal and an extraction method, the extract contains genistin, daidzin and daidzein, and the product provided by the method is A mixture of isoflavone glycosides, and there is no separation method for genistin. At present, there is an urgent need to develop a method for extracting high-purity genistin suitable for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 彭开锋张鹏龚云刘逆夫
Owner ZHUZHOU QIANJIN PHARMA
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