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Refined part of total gardenia crocin with anti-depression effect as well as preparation method and application of refined part

A technology of crocin and anti-depression, which is applied in the field of refined parts of gardenia total crocin, to achieve the effect of good treatment of depression, less toxic and side effects, and good application prospects

Inactive Publication Date: 2015-04-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the application of the total crocin extract of Gardenia jasminoides in the preparation process of medicines or health care products for the treatment or prevention of depression has not been reported yet.

Method used

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  • Refined part of total gardenia crocin with anti-depression effect as well as preparation method and application of refined part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 gardenia crocin part

[0027] Take 1 kg of gardenia medicinal material, crush it, add 8 times the amount of 50% ethanol to reflux and extract 3 times, each time for 1 hour, combine the extracts, filter, concentrate the extracts under reduced pressure until there is no alcohol smell, filter, and filter the filtrate through D101 Porous resin column, all the medicinal liquid passes through the macroporous resin column, washes with distilled water to remove impurities, and then elutes with 10 times the column volume of 50% ethanol, collects the eluate, concentrates under reduced pressure to a relative density of 1.3-1.5, and concentrates Pass through the Sephadex LH-20 Sephadex column, wash with 10% ethanol first, remove non-crocin components, then elute with 50% ethanol with 5 times the volume of the column, collect the eluate, concentrate under reduced pressure and evaporate to dryness , that is, the total saffron fraction.

[0028] The ca...

Embodiment 2

[0032] The preparation of embodiment 2 gardenia crocin parts

[0033] Take 1 kg of gardenia medicinal material, crush it, add 12 times the amount of 50% ethanol to reflux and extract 3 times, each time for 2 hours, combine the extracts, filter, concentrate the extracts under reduced pressure until there is no alcohol smell, filter, and filter the filtrate through D101 Porous resin column, the drug solution is washed with distilled water after passing through the macroporous resin column to remove impurities, and then eluted with 12 times the column volume of 50% ethanol, the eluate is collected, concentrated under reduced pressure to a relative density of 1.3-1.5, and the concentrated solution Pass through the Sephadex LH-20 Sephadex column, wash with 10% ethanol first, remove non-crocin components, then elute with 8 times the column volume of 50% ethanol, collect the eluate, concentrate under reduced pressure and evaporate to dryness , that is, the total saffron fraction.

...

Embodiment 3

[0038] Example 3 Experiment of treatment of chronic unpredictable stress depression with total crocin parts of Gardenia jasminoides

[0039] 1. Materials and methods

[0040] 1. Drugs: Positive drug: Fluoxetine hydrochloride capsules are products of Eli Lilly and Company of the United States, product batch number: J20080016. Test drug: the gardenia crocin extract prepared in Example 1 of the present invention.

[0041]2. Modeling: Except for the blank group, mice in other groups received a stimulus for a total of 21 days, including fasting for 24 hours, wet cage for 24 hours, restraint for 6 hours, water deprivation for 24 hours, day and night reversed, cage tilting for 24 hours, random every day Choose 1 stimulus.

[0042] 3. Grouping and administration:

[0043] Sixty Kunming mice were fed for one week to adapt to the environment, and were randomly divided into 6 groups. Except for the blank group, the mice in the other groups were housed in single cages. After 21 days o...

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Abstract

The invention discloses a refined part of total gardenia crocin with an anti-depression effect. The optimal process for preparing the refined part of the total gardenia crocin screened by a plurality of experiments adopts a method combining a macroporous resin column and a sephadex column to separate and prepare the refined part of the total gardenia crocin with relatively high purity. An experiment result shows that the refined part has a good effect of treating the depressive disorder; the acting time is obviously faster than that of positive medicine fluoxetine hydrochloride; the action effect is higher than that of fluoxetine; and the action time of the fluoxetine needs over three weeks in general. The clinical experiment result shows that the crocin is nontoxic, free of addiction and small in toxic and side effects, has a good application prospect, and is expected to be developed into novel purely natural anti-depression medicines or health products.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a refined part of gardenia total crocin with clear curative effect, high safety and antidepressant effect and an application thereof. Background technique [0002] Depression is a disease characterized by significant and persistent low mood, accompanied by insomnia, memory loss, eating disorders and other symptoms. In severe cases, suicidal thoughts and behaviors may appear. It has been reported that 16% of the population will experience symptoms of depression at some point in their lives. If the patient has these symptoms for more than two weeks and does not improve significantly, it can be diagnosed as depression. According to the statistics of the World Health Organization, depression has become the fourth largest disease in the world; and it is estimated that by 2020, it may become the second largest disease after coronary heart d...

Claims

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Application Information

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IPC IPC(8): A61K36/744A23L1/29A61P25/24A23L33/00
CPCA61K36/744A23V2002/00A61K2236/33A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23V2200/30A23V2200/322A23V2250/21
Inventor 陶伟伟陈刚
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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