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Hypertension susceptibility relevant gene variation locus and detecting method thereof

A technology for hypertension and essential hypertension, applied in the fields of molecular biology and medicine, can solve the problems of abnormal expression regulation, less research, and decreased expression, etc.

Inactive Publication Date: 2015-03-25
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

[0006] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

Method used

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  • Hypertension susceptibility relevant gene variation locus and detecting method thereof

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Experimental program
Comparison scheme
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Embodiment 1

[0035] The -35 site of the 5' non-coding region of the essential hypertension susceptibility gene MFn2 gene was detected by sequencing. 30 cases of each of the above-mentioned hypertension cases and control groups were selected for sequencing to determine the genotype of the 5' UTR-35.

[0036] 1. Experimental method

[0037] The above PCR primers were still used as primers for PCR sequencing, and the amplified products were directly sequenced after purification. The sequencing instrument is ABI's 3130xl genetic analyzer, which is analyzed with sequence analysis 5.2 analysis software, and the results can also be viewed with chromas.

[0038] 2. Experimental results

[0039] The screenshot of the sequencing result is as follows: figure 1 shown.

[0040]

[0041] All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. In addition, it should be understood that after reading the abo...

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Abstract

The invention discloses a primary hypertension susceptibility relevant single-nucleotide locus and a method for detecting the single-nucleotide polymorphism of a primary hypertension relevant gene-mitofusin 2 gene(Mfn2 gene) / / hyperplasia suppressor gene (HSG), wherein the detecting method comprises detecting the genotypes of basic groups between -945bp and -946bp in a non-coding region at the 5' end of the individual mitofusin 2 (Mfn2) / hyperplasia suppressor gene (HSG). The invention also discloses a corresponding detecting kit.

Description

[0001] technical field [0002] The present invention relates to the fields of molecular biology and medicine. More specifically, it involves the detection of the 5' non-coding region-35bp of the single nucleotide polymorphism (single nucleotide polymorphism, SNP) site of the human mitochondrial fusion gene 2 (Mitofusin 2, Mfn2 gene). The invention also relates to a kit for detecting the SNP site. Background technique [0003] Essential hypertension (essential hypertension, EH) is a common and frequent multifactorial and polygenic disease caused by both environmental and genetic factors, which has had a great impact on human health. With the development of molecular medicine, more than 150 hypertension-related genes have been discovered, but the pathogenesis of EH is still not completely clear, and the early diagnosis and prevention of hypertension have not been fully resolved. EH is the result of the joint action of genetic factors and the environment, and 30%-60% of chan...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 王佐广魏永祥李梅刘雅牛秋丽彭晓云刘洁琳马晓海李继勇温绍君曾荣
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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