Biological pesticide-added fertilizer and preparation method thereof
A biomedical fertilizer and functional microorganism technology, applied in the field of medicinal fertilizer and preparation, can solve the problems of chemical fertilizers destroying soil physical and chemical properties, enhancing disease and insect resistance, and destroying ecology, etc., to improve soil physical and chemical properties, high-efficiency utilization, and comprehensive nutrition Effect
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Embodiment 1
[0031] The Bacillus amyloliquefaciens and the decomposing compound bacterial agent in this example all come from the Pollution-free Pesticide Research Service Center of Yangling Agricultural Science and Technology University. The Houji brand compound fertilizer produced by Huayang Chemical Co., Ltd. specifically includes N-P 2 o 5 -K 2 The ratio of O is 16:16:16, the total nutrient content is ≧48%,
[0032] Bacillus amyloliquefaciens culture medium: nutrient broth medium, without adding agar as a liquid medium, adding 1.5% agar as a solid medium; the formula of the nutrient broth medium is: beef extract 5g / L, peptone 10g / L and NaCl 5g / L; decomposed compound microbial culture medium: modified Gaoshi No. 1 medium, without adding agar as a liquid medium, adding 1.5% agar as a solid medium; the formula of the improved Gaoshi No. 1 medium is: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.01g, 0.5g NaCl, 1000mL distilled water, pH 7.2~7.4.
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Embodiment 2
[0044] The Bacillus amyloliquefaciens and the decomposing compound bacterial agent in this example all come from the Pollution-free Pesticide Research Service Center of Yangling Agricultural Science and Technology University. The Houji brand compound fertilizer produced by Huayang Chemical Co., Ltd. specifically includes N-P 2 o 5 -K 2 O ratio is 16:16:16, total nutrient content ≧48%
[0045] Bacillus amyloliquefaciens culture medium: nutrient broth medium, liquid medium without agar, solid medium with 1.5% agar; the formula of nutrient broth medium is beef extract 5g / L, peptone 10g / L, NaCl 5g / L.
[0046] Decomposed compound bacterial agent medium: improved Gaoshi No. 1 medium, without adding agar as a liquid medium, adding 1.5% agar as a solid medium; the formula of the improved Gaoshi No. 1 medium is: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.01g, 0.5g NaCl, 1000mL distilled water, pH 7.2~7.4.
[0047] Inoculate Bacillus amylolique...
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